Human Dopachrome Tautomerase ELISA Kit | DT elisa kit
Human Dopachrome Tautomerase ELISA Kit
Gene Names
DCT; TRP-2; TYRP2
Reactivity
Human
Synonyms
Dopachrome Tautomerase; Human Dopachrome Tautomerase ELISA Kit; DT elisa kit
Reactivity
Human
Specificity
No significant cross-reactivity or interference between Human DT and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
0.625 ng/ml - 20 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated DT concentrations in Human serum, plasma and other body fluids. Using Purified Human DT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DT and DT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for DT elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated DT concentrations in Human serum, plasma and other body fluids. Using Purified Human DT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DT and DT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
59,145 Da
NCBI Official Full Name
dopachrome tautomerase, partial
NCBI Official Synonym Full Names
dopachrome tautomerase
NCBI Official Symbol
DCT
NCBI Official Synonym Symbols
TRP-2; TYRP2
NCBI Protein Information
L-dopachrome tautomerase; DT; TRP2; dopachrome delta-isomerase; tyrosine-related protein 2; L-dopachrome Delta-isomerase; tyrosinase-related protein 2; dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
UniProt Protein Name
L-dopachrome tautomerase
UniProt Gene Name
DCT
UniProt Synonym Gene Names
TYRP2; DCT; DT; TRP-2; TRP2
UniProt Entry Name
TYRP2_HUMAN
Uniprot Description
DCT: Involved in regulating eumelanin and phaeomelanin levels. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell development/differentiation; Isomerase; Amino Acid Metabolism - tyrosine; EC 5.3.3.12
Chromosomal Location of Human Ortholog: 13q32
Cellular Component: melanosome membrane; melanosome; integral to membrane; cytosol
Molecular Function: copper ion binding; dopachrome isomerase activity; oxidoreductase activity
Biological Process: neuroblast division in the ventricular zone; positive regulation of neuroblast proliferation; epidermis development; pigmentation during development; melanin biosynthetic process from tyrosine; cell development
Research Articles on DT
Similar Products
Product Notes
The Human DT dct (Catalog #AAA008611) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA008611 ELISA Kit recognizes Human DT. It is sometimes possible for the material contained within the vial of "Dopachrome Tautomerase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
