Rabbit MAP2K3-S189 Polyclonal Antibody | anti-MAP2K3-S189 antibody

Phospho-MAP2K3-S189 Polyclonal Antibody

Cat. Num. AAA8527444

• Gene Names: MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: MAP2K3-S189; Polyclonal Antibody; Phospho-MAP2K3-S189 Polyclonal Antibody; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2; anti-MAP2K3-S189 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 318

• Applicable Applications for anti-MAP2K3-S189 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A phospho specific peptide corresponding to residues surrounding S189 of human MAP2K3
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-MAP2K3-S189 antibody

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4, 5). Activation of MKK3 and MKK6 occurs through phosphorylation of Ser189 and Thr193 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4, 5).

References

1. Derijard, B. et al. (1995) Science 267, 682-685. 2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426. 3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384. 4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255. 5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

NCBI and Uniprot Product Information

• NCBI GI #: 938403439
• NCBI GeneID: 5606
• NCBI Accession #: NP_001303261.1
• NCBI GenBank Nucleotide #: NM_001316332.1
• UniProt Accession #: P46734; Q99441; Q9UE71; Q9UE72; B3KSK7
• Molecular Weight: 39,940 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 3 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 3
• NCBI Official Symbol: MAP2K3
• NCBI Official Synonym Symbols: MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 3
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 3
• UniProt Gene Name: MAP2K3
• UniProt Synonym Gene Names: MEK3; MKK3; PRKMK3; SKK2; MAP kinase kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; SAPKK-2; SAPKK2

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.