PKN1 sirna

PKN1 siRNA (Human)

Cat. Num. AAA8235509

• Gene Names: PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PKN1; PKN1 siRNA (Human); PAK1; PKN; PRK1; PRKCL1; Serine/threonine-protein kinase N1; Protease-activated kinase 1; PAK-1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N; PKN1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PKN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 942

• Applicable Applications for PKN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PKN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PKN1 sirna

siRNA to inhibit PKN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 47132589
• NCBI GeneID: 5585
• NCBI Accession #: NP_002732.3
• NCBI GenBank Nucleotide #: NM_002741.3
• UniProt Accession #: Q16512; Q15143; Q504U4; Q8IUV5; Q9UD44; A8K7W5; B2R9R4; B3KVN3
• Molecular Weight: 66,060 Da
• NCBI Official Full Name: serine/threonine-protein kinase N1 isoform 2
• NCBI Official Synonym Full Names: protein kinase N1
• NCBI Official Symbol: PKN1
• NCBI Official Synonym Symbols: DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• NCBI Protein Information: serine/threonine-protein kinase N1
• UniProt Protein Name: Serine/threonine-protein kinase N1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PKN1
• UniProt Synonym Gene Names: PAK1; PKN; PRK1; PRKCL1; PAK-1
• UniProt Entry Name: PKN1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PKN1: an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; PKN family

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; midbody; nucleus; endosome; cleavage furrow

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein kinase C binding; histone binding; histone deacetylase binding; Rac GTPase binding; GTP-Rho binding; chromatin binding; ATP binding; protein kinase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; hyperosmotic response; transcription, DNA-dependent; signal transduction; protein amino acid phosphorylation; activation of JNK activity