GPER1 sirna

GPER1 siRNA (Rat)

Cat. Num. AAA8226241

• Gene Names: Gper1; Gper; GPR41; Gpr30
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GPER1; GPER1 siRNA (Rat); CMKRL2; GPER; GPR30; GPR41; G-protein coupled estrogen receptor 1; Chemoattractant receptor-like 2; G protein-coupled estrogen receptor 1; G-protein coupled receptor 30; G-protein coupled receptor 41; Membrane estrogen receptor; mER; GPER1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GPER1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 375

• Applicable Applications for GPER1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GPER1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GPER1 sirna

siRNA to inhibit GPER1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19424262
• NCBI GeneID: 171104
• NCBI Accession #: NP_598257.1
• NCBI GenBank Nucleotide #: NM_133573.1
• UniProt Accession #: O08878
• Molecular Weight: 42,260 Da
• NCBI Official Full Name: G-protein coupled estrogen receptor 1
• NCBI Official Synonym Full Names: G protein-coupled estrogen receptor 1
• NCBI Official Symbol: Gper1
• NCBI Official Synonym Symbols: Gper; GPR41; Gpr30
• NCBI Protein Information: G-protein coupled estrogen receptor 1
• UniProt Protein Name: G-protein coupled estrogen receptor 1
• Protein Family: G-protein coupled estrogen receptor
• UniProt Gene Name: Gper1
• UniProt Synonym Gene Names: Cmkrl2; Gper; Gpr30; Gpr41; mER
• UniProt Entry Name: GPER1_RAT

NCBI Description

an orphan G protein-coupled receptor found in lung [RGD, Feb 2006]

Uniprot Description

GPER1: Receptor for estrogen. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Cellular Component: Golgi apparatus; basolateral plasma membrane; early endosome; dendrite; integral to membrane; nuclear envelope; trans-Golgi network; cytosol; postsynaptic membrane; recycling endosome; perinuclear region of cytoplasm; keratin filament; cytoplasm; dendritic shaft; intracellular; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; endoplasmic reticulum; postsynaptic density; Golgi membrane; presynaptic membrane; axon; mitochondrial membrane; presynaptic active zone; plasma membrane; nerve terminal; cell junction; nucleus

Molecular Function: G-protein coupled receptor activity; estrogen receptor activity; hormone binding; steroid hormone receptor activity; mineralocorticoid receptor activity; drug binding; chromatin binding; steroid binding

Biological Process: positive regulation of apoptosis; generation of action potential; positive regulation of caspase activity; nuclear fragmentation during apoptosis; positive regulation of vasodilation; negative regulation of DNA metabolic process; positive regulation of neurotransmitter secretion; negative regulation of leukocyte activation; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; inflammatory response; steroid hormone receptor signaling pathway; positive regulation of neurogenesis; cytosolic calcium ion homeostasis; negative regulation of lipid biosynthetic process; positive regulation of insulin secretion; positive regulation of inositol trisphosphate biosynthetic process; apoptotic chromosome condensation; negative regulation of fat cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; mineralocorticoid receptor signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; innate immune response; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of protein amino acid phosphorylation; positive regulation of cell migration