NUMBL sirna

NUMBL siRNA (Human)

Cat. Num. AAA8224195

• Gene Names: NUMBL; NBL; CAG3A; CTG3a; NUMBR; NUMB-R; TNRC23; NUMBLIKE
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUMBL; NUMBL siRNA (Human); Numb-like protein; Numb-related protein; Numb-R; NUMBL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NUMBL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 568

• Applicable Applications for NUMBL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NUMBL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUMBL sirna

siRNA to inhibit NUMBL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 586798132
• NCBI GeneID: 9253
• NCBI Accession #: NP_001276908.1
• NCBI GenBank Nucleotide #: NM_001289979.1
• UniProt Accession #: Q9Y6R0; Q7Z4J9
• Molecular Weight: 64,891 Da
• NCBI Official Full Name: numb-like protein isoform b
• NCBI Official Synonym Full Names: numb homolog (Drosophila)-like
• NCBI Official Symbol: NUMBL
• NCBI Official Synonym Symbols: NBL; CAG3A; CTG3a; NUMBR; NUMB-R; TNRC23; NUMBLIKE
• NCBI Protein Information: numb-like protein
• UniProt Protein Name: Numb-like protein
• Protein Family: Numb-like protein
• UniProt Gene Name: NUMBL
• UniProt Synonym Gene Names: Numb-R
• UniProt Entry Name: NUMBL_HUMAN

Uniprot Description

NUMBL: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF- kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons. Interacts (via PTB domain) with MAP3K7IP2 (via C- terminal). Interacts (via C-terminal) with TRAF6 (via TRAF domains). Associates with EPS15 and NOTCH1.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13.13-q13.2

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: nervous system development; positive regulation of neurogenesis; axonogenesis; cytokine and chemokine mediated signaling pathway; protein metabolic process; neuroblast division in the subventricular zone; positive regulation of dendrite morphogenesis; lateral ventricle development