FOXN1 sirna

FOXN1 siRNA (Mouse)

Cat. Num. AAA8222202

• Gene Names: Foxn1; nu; Whn; nude; Fkh19; Hfh11; HFH-11; D11Bhm185e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FOXN1; FOXN1 siRNA (Mouse); FKH19; HFH11; WHN; Forkhead box protein N1; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HNF-3/forkhead homolog 11; Winged-helix transcription factor nude; FOXN1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FOXN1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 648

• Applicable Applications for FOXN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FOXN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FOXN1 sirna

siRNA to inhibit FOXN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6680211
• NCBI GeneID: 15218
• NCBI Accession #: NP_032264.1
• NCBI GenBank Nucleotide #: NM_008238.2
• UniProt Accession #: Q61575
• Molecular Weight: 69,245 Da
• NCBI Official Full Name: forkhead box protein N1 isoform 1
• NCBI Official Synonym Full Names: forkhead box N1
• NCBI Official Symbol: Foxn1
• NCBI Official Synonym Symbols: nu; Whn; nude; Fkh19; Hfh11; HFH-11; D11Bhm185e
• NCBI Protein Information: forkhead box protein N1
• UniProt Protein Name: Forkhead box protein N1
• Protein Family: Forkhead box protein
• UniProt Gene Name: Foxn1
• UniProt Synonym Gene Names: Fkh19; Hfh11; Whn; HFH-11; HNF-3/forkhead homolog 11
• UniProt Entry Name: FOXN1_MOUSE

NCBI Description

The protein encoded by this gene is part of the forkhead family or "winged-helix" transcription factors that are important in developmental processes, immune system regulation, metabolism, cancer and aging. This gene family has over 100 members, subdivided into classes (A-Q) based on phylogeny. The encoded protein is proposed to regulate development of the thymus and differentiation of keratinocytes. Mutations in this gene cause severe primary T-cell immunodeficiency and congenital alopecia. In mouse mutations of this gene underlie the phenotype of the nude mouse, which has been widely used as a model system in oncology, immunology, dermatology, and transplantation studies. In humans mutations in this gene have been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]

Uniprot Description

FOXN1: Transcriptional regulator involved in development. Defects in FOXN1 are the cause of T-cell immunodeficiency congenital alopecia and nail dystrophy (TIDAND). A disorder characterized by the association of congenital alopecia, severe T-cell immunodeficiency, and ridging and pitting of all nails.

Protein type: DNA-binding; Transcription factor; Cell development/differentiation

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; epidermis development; transcription, DNA-dependent; thymus development; regulation of T cell differentiation in the thymus; multicellular organismal development; keratinocyte differentiation; positive regulation of hair follicle development; regulation of transcription from RNA polymerase II promoter; cell proliferation; epithelial cell proliferation; T cell lineage commitment; hair follicle development; regulation of transcription, DNA-dependent; regulation of gene expression; blood vessel morphogenesis; hemopoiesis; T cell homeostasis; lymphocyte homeostasis