UCN sirna

UCN siRNA (Human)

Cat. Num. AAA8221398

• Gene Names: UCN; UI; UROC
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UCN; UCN siRNA (Human); Urocortin; UCN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UCN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 124

• Applicable Applications for UCN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UCN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UCN sirna

siRNA to inhibit UCN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4507803
• NCBI GeneID: 7349
• NCBI Accession #: NP_003344.1
• NCBI GenBank Nucleotide #: NM_003353.2
• UniProt Accession #: P55089; Q6FG64
• Molecular Weight: 13,458 Da
• NCBI Official Full Name: urocortin preproprotein
• NCBI Official Synonym Full Names: urocortin
• NCBI Official Symbol: UCN
• NCBI Official Synonym Symbols: UI; UROC
• NCBI Protein Information: urocortin
• UniProt Protein Name: Urocortin
• Protein Family: Urocortin
• UniProt Gene Name: UCN
• UniProt Entry Name: UCN1_HUMAN

NCBI Description

This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. [provided by RefSeq, Jul 2008]

Uniprot Description

UCN: Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: dendrite; extracellular region; perikaryon; nerve terminal

Molecular Function: corticotropin-releasing hormone receptor 1 binding; histone deacetylase inhibitor activity; neuropeptide hormone activity; protein binding; corticotropin-releasing hormone receptor 2 binding

Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of translation; negative regulation of hormone secretion; positive regulation of collagen biosynthetic process; response to glucocorticoid stimulus; drinking behavior; negative regulation of cell size; response to pain; female pregnancy; positive regulation of vasodilation; pancreatic juice secretion; negative regulation of appetite; response to estradiol stimulus; positive regulation of adrenocorticotropic hormone secretion; sensory perception of sound; neuropeptide signaling pathway; positive regulation of cAMP biosynthetic process; negative regulation of blood pressure; inflammatory response; neurite development; associative learning; negative regulation of histone deacetylation; activation of protein kinase A; positive regulation of interleukin-6 production; social behavior; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of vascular permeability; G-protein coupled receptor protein signaling pathway; aerobic respiration; response to oxidative stress; positive regulation of DNA replication; negative regulation of apoptosis