KCNE1 sirna

KCNE1 siRNA (Rat)

Cat. Num. AAA8210911

• Gene Names: Kcne1; m; Isk
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNE1; KCNE1 siRNA (Rat); Potassium voltage-gated channel subfamily E member 1; Delayed rectifier potassium channel subunit IsK; IKs producing slow voltage-gated potassium channel subunit beta Mink; Minimal potassium channel; KCNE1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KCNE1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 130

• Applicable Applications for KCNE1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KCNE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNE1 sirna

siRNA to inhibit KCNE1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981124
• NCBI GeneID: 25471
• NCBI Accession #: NP_037105.1
• NCBI GenBank Nucleotide #: NM_012973.1
• UniProt Accession #: P15383
• Molecular Weight: 14,699 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily E member 1
• NCBI Official Synonym Full Names: potassium channel, voltage-gated Isk-related subfamily E regulatory beta subunit 1
• NCBI Official Symbol: Kcne1
• NCBI Official Synonym Symbols: m; Isk
• NCBI Protein Information: potassium voltage-gated channel subfamily E member 1
• UniProt Protein Name: Potassium voltage-gated channel subfamily E member 1
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: Kcne1
• UniProt Entry Name: KCNE1_RAT

NCBI Description

beta subunit of the voltage-gated potassium channel complex; involved in modulating channel kinetics [RGD, Feb 2006]

Uniprot Description

KCNE1: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Associates with KCNQ1/KVLQT1 and KCNH2/HERG. Expressed in heart, lung, kidney, testis, ovaries, small intestine, peripheral blood leukocytes. Not detected in pancreas, spleen, prostate and colon. Restrictively localized in the apical membrane portion of epithelial cells. Belongs to the potassium channel KCNE family.

Protein type: Channel, potassium; Membrane protein, integral

Cellular Component: Golgi apparatus; voltage-gated potassium channel complex; cell surface; endoplasmic reticulum; lysosome; apical plasma membrane; plasma membrane; Z disc

Molecular Function: protein binding; voltage-gated potassium channel activity; potassium channel regulator activity; telethonin binding; protein heterodimerization activity; delayed rectifier potassium channel activity

Biological Process: protein amino acid O-linked glycosylation; heart contraction; epithelial cell maturation; male gonad development; protein amino acid N-linked glycosylation