OX40L/TNFSF4 Lysate | TNFSF4 lysate

Mouse OX40L/TNFSF4 HEK293 Overexpression Lysate

Cat. Num. AAA8119756

• Applications: Western Blot
• Synonyms: OX40L/TNFSF4; Mouse OX40L/TNFSF4 HEK293 Overexpression Lysate; Mouse OX40L/TNFSF4 HEK293 Cell Lysate (WB positive control); Mouse Ath-1 Overexpression Lysate; Mouse Ath1 Overexpression Lysate; Mouse CD134L Overexpression Lysate; Mouse gp34 Overexpression Lysate; Mouse OX-40L Overexpression Lysate; Mouse Ox40l Overexpression Lysate; Mouse TXGP1 Overexpression Lysate; Mouse Txgp1l Overexpression Lysate; tumor necrosis factor (ligand) superfamily; member 4; TNFSF4 lysate

• Host: HEK293 Cells
• Form/Format: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.; 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

• Applicable Applications for TNFSF4 lysate: Western Blot (WB)
• Application Notes: Western blot (WB): Use at an assay dependent dilution.
• Species: Mouse
• Sequence Information: A DNA sequence encoding the mouse TNFSF4 (NP_033478.1)(Ser51-Leu198) was expressed with a polyhistidine tag at the C-terminus.
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Recommend Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.; 2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.; 3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
• Preparation and Storage: Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.

Related Product Information for TNFSF4 lysate

Description: This Mouse OX40L/TNFSF4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of OX40L/TNFSF4 protein from the overexpression lysate was verified.

Background: HEK293 Cell Mouse OX40L/TNFSF4 transfected/overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX40. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD4-stimulated B cells, this cytokine along with CD7 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.

Product Categories/Family for TNFSF4 lysate

Cancer Drug Targets

References

Lei W. et al., 2012, Ann Acad Med Singapore. 41 (5): 200-4. Lee YH. et al., 2012, Hum Immunol. 73 (10): 1050-4. Weiguang Y. et al., 2012, PLoS One. 7 (8): e41277.