Carbonic Anhydrase X Cell Lysate | CA10 cell lysate
Mouse Carbonic Anhydrase X HEK293 Overexpression Lysate
Gene Names
Car10; Ca10; BB085816; 2700029L05Rik
Applications
Western Blot
Synonyms
Carbonic Anhydrase X; Mouse Carbonic Anhydrase X HEK293 Overexpression Lysate; Mouse Carbonic Anhydrase X/Car10 HEK293 Cell Lysate (WB positive control); carbonic anhydrase X; Mouse 2700029L05Rik Overexpression Lysate; Mouse BB085816 Overexpression Lysate; Mouse Ca10 Overexpression Lysate; Mouse Car10 Overexpression Lysate; Mouse RP23-448I9.1 Overexpression Lysate; CA10 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
289aa
Sequence
Met1-Asn300
Applicable Applications for CA10 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse Car10 (P61215) (Met 1-Asn 300) was expressed, fused with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CA10 cell lysate
Carbonic anhydrase X, also called carbonic anhydrase-related protein X (CARPX) and CA1, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
This Mouse Carbonic Anhydrase X overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Carbonic Anhydrase X protein from the overexpression lysate was verified.
This Mouse Carbonic Anhydrase X overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Carbonic Anhydrase X protein from the overexpression lysate was verified.
References
Okamoto, N. et al., 2001, Biochim. Biophys. Acta 1518:311-316. Taniuchi, K. et al., Neuroscience 112: 93-99. Supuran, C.T. et al., 2003, Med. Res. Rev. 23: 146-189.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,563 Da
NCBI Official Full Name
carbonic anhydrase-related protein 10
NCBI Official Synonym Full Names
carbonic anhydrase 10
NCBI Official Symbol
Car10
NCBI Official Synonym Symbols
Ca10; BB085816; 2700029L05Rik
NCBI Protein Information
carbonic anhydrase-related protein 10
UniProt Protein Name
Carbonic anhydrase-related protein 10
Protein Family
UniProt Gene Name
Ca10
UniProt Synonym Gene Names
Car10; CA-RP X; CARP X
Uniprot Description
CA10: Does not have a catalytic activity. Belongs to the alpha-carbonic anhydrase family.
Chromosomal Location of Human Ortholog: 11|11 D
Research Articles on CA10
Similar Products
Product Notes
The CA10 ca10 (Catalog #AAA8116859) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 289aa. AAA Biotech's Carbonic Anhydrase X can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CA10 ca10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asn30 0. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase X, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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