Meprin alpha/MEP1A Cell Lysate | MEP1A cell lysate
Mouse Meprin alpha/MEP1A HEK293 Overexpression Lysate
Gene Names
Mep1a; Mep1; Mep-1; Mep-1a; AI098089; AW107200
Applications
Western Blot
Synonyms
Meprin alpha/MEP1A; Mouse Meprin alpha/MEP1A HEK293 Overexpression Lysate; Mouse MEP1A HEK293 Cell Lysate (WB positive control); meprin A; alpha (PABA peptide hydrolase); Mouse AI098089 Overexpression Lysate; Mouse AW107200 Overexpression Lysate; Mouse Mep-1 Overexpression Lysate; Mouse Mep-1a Overexpression Lysate; Mouse Mep1 Overexpression Lysate; MEP1A cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
606aa
Sequence
Met1-Arg615
Applicable Applications for MEP1A cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse MEP1A (NP_032611.2) (Met 1-Arg 615) was expressed, fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for MEP1A cell lysate
Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I membrane protein that belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo-and hetero-oligomeric enzymes. Meprins are abundantly expressed in the kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials, and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury (AKI).
This Mouse Meprin alpha/MEP1A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Meprin alpha/MEP1A protein from the overexpression lysate was verified.
This Mouse Meprin alpha/MEP1A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Meprin alpha/MEP1A protein from the overexpression lysate was verified.
References
Bertenshaw, GP. et al., 2002, Biol Chem. 383 (7-8):1175-83. Bond, JS. et al., 2005, FEBS Lett. 579 (15): 3317-22. Herzog, C. et al., 2007, Kidney Int. 71 (10): 1009-18. Yura, RE. et al., 2009, Am J Physiol Renal Physiol. 296 (1): F135-44.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,231 Da
NCBI Official Full Name
meprin A subunit alpha
NCBI Official Synonym Full Names
meprin 1 alpha
NCBI Official Symbol
Mep1a
NCBI Official Synonym Symbols
Mep1; Mep-1; Mep-1a; AI098089; AW107200
NCBI Protein Information
meprin A subunit alpha; meprin alpha; endopeptidase-2; meprin A alpha-subunit
UniProt Protein Name
Meprin A subunit alpha
Protein Family
UniProt Gene Name
Mep1a
UniProt Entry Name
MEP1A_MOUSE
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Product Notes
The MEP1A mep1a (Catalog #AAA8116334) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 606aa. AAA Biotech's Meprin alpha/MEP1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the MEP1A mep1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Arg61 5. It is sometimes possible for the material contained within the vial of "Meprin alpha/MEP1A, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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