GPR37 cell lysate
Human GPR37 HEK293 Overexpression Lysate
Gene Names
GPR37; PAELR; EDNRBL; hET(B)R-LP
Applications
Western Blot
Synonyms
GPR37; Human GPR37 HEK293 Overexpression Lysate; Human GPR37 HEK293 Cell Lysate (WB positive control); G protein-coupled receptor 37 (endothelin receptor type B-like); Human EDNRBL Overexpression Lysate; Human hET(B)R-LP Overexpression Lysate; Human PAELR Overexpression Lysate; GPR37 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
250aa
Sequence
Met1-Met265
Applicable Applications for GPR37 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human GPR37 (NP_005293.1) the first extracellular domaqin (Met 1-Met 265) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for GPR37 cell lysate
GPR37 (cathepsin Z) is an orphan receptor that belongs to the G-protein coupled receptor 1 family. G protein-coupled receptors are a large protein family comprised of transmembrane receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. They only exist in eukaryotes, including yeast, choanoflagellates, and animals. These receptors are bonded and activated by light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters. These ligands vary in size from small molecules to peptides to large proteins. G protein-coupled receptors are involved in many diseases and are also the target of approximately 40% of all modern medicinal drugs. GPR37 is expressed in the brain and spinal cord, and at lower levels in the testis, placenta, and liver, but no detectable expression is observed in any other tissue. GPR37 may have a unique functional role in the central nervous system.
This Human GPR37 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPR37 protein from the overexpression lysate was verified.
This Human GPR37 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPR37 protein from the overexpression lysate was verified.
References
Marazziti D, et al. (1997) Cloning of GPR37, a gene located on chromosome 7 encoding a putative G-protein-coupled peptide receptor, from a human frontal brain EST library. Genomics. 45(1):68-77.Imai, et al. (2002) CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity. Mol Cell. 10(1):55-67.Imai, et al. (2001) An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin. Cell. 105(7):891-902.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,114 Da
NCBI Official Full Name
probable G-protein coupled receptor 37
NCBI Official Synonym Full Names
G protein-coupled receptor 37 (endothelin receptor type B-like)
NCBI Official Symbol
GPR37
NCBI Official Synonym Symbols
PAELR; EDNRBL; hET(B)R-LP
NCBI Protein Information
probable G-protein coupled receptor 37; ETBR-LP-1; endothelin B receptor-like protein 1; Parkin-associated endothelin receptor-like receptor
UniProt Protein Name
Probable G-protein coupled receptor 37
Protein Family
UniProt Gene Name
GPR37
UniProt Synonym Gene Names
ETBR-LP-1; PAELR
UniProt Entry Name
GPR37_HUMAN
NCBI Description
This gene is a member of the G protein-coupled receptor family. The encoded protein contains seven transmembrane domains and is found in cell and endoplasmic reticulum membranes. G protein-coupled receptors are involved in translating outside signals into G protein mediated intracellular effects. This gene product interacts with Parkin and is involved in juvenile Parkinson disease. [provided by RefSeq, Oct 2012]
Uniprot Description
GPR37: Orphan receptor. May have a unique functional role in the central nervous system. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: 7q31
Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; ubiquitin ligase complex; receptor complex
Molecular Function: G-protein coupled receptor activity; protein binding; ubiquitin protein ligase binding; heat shock protein binding; peptide receptor activity, G-protein coupled; Hsp70 protein binding; peptide binding
Biological Process: positive regulation of dopamine metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of MAPKKK cascade; G-protein signaling, adenylate cyclase inhibiting pathway; dopamine biosynthetic process; locomotion during locomotory behavior
Research Articles on GPR37
Similar Products
Product Notes
The GPR37 gpr37 (Catalog #AAA8115178) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 250aa. AAA Biotech's GPR37 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the GPR37 gpr37 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Met26 5. It is sometimes possible for the material contained within the vial of "GPR37, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
