IGSF11 cell lysate
Human IGSF11 HEK293 Overexpression Lysate
Gene Names
IGSF11; CT119; VSIG3; Igsf13; BT-IgSF; CXADRL1
Applications
Western Blot
Synonyms
IGSF11; Human IGSF11 HEK293 Overexpression Lysate; Human IGSF11/BTIGSF HEK293 Cell Lysate (WB positive control); immunoglobulin superfamily; member 11; Human BT-IgSF Overexpression Lysate; Human CT119 Overexpression Lysate; Human CXADRL1 Overexpression Lysate; Human Igsf13 Overexpression Lysate; Human VSIG3 Overexpression Lysate; IGSF11 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
229aa
Sequence
Met1-Gly240
Applicable Applications for IGSF11 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain of human IGSF11 (AAH34411.1) (Met 1-Gly 240) was fused with a polyhistidine tag at the C-terminus
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for IGSF11 cell lysate
Immunoglobulin superfamily member 11(IGSF11) is expressed on the plasma membrane in the testis and brain. These IGSF proteins undergo final modifications during capacitation and/or the acrosome reaction. IGSF proteins share significant homology with endothelial cell-selective adhesion molecule and coxsackievirus and adenovirus receptor, which mediates cell attachment and homotypic intercellular interactions. In clinical, the IGSF11 has been reported to overexpressed in colorectal cancers and hepatocellular carcinomas, as well as intestinal-type gastric cancers, compared to their corresponding non-cancerous tissues. The IGSF11 has also been found expressed abundantly in the testis and ovary and the IGSF11 can be used as a candidate of cancer-testis antigen.
This Human IGSF11 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IGSF11 protein from the overexpression lysate was verified.
This Human IGSF11 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IGSF11 protein from the overexpression lysate was verified.
References
Toshimori K, et al. (2006) The involvement of immunoglobulin superfamily proteins in spermatogenesis and sperm-egg interaction. Reproductive Medicine and Biology. 5(2): 87-93.Coyne CB, et al. (2005) CAR: A virus receptor within the tight junction. Advanced Drug Delivery Reviews. 57(6) : 869-82.Watanabe T, et al. (2005) Identification of immunoglobulin superfamily 11 (IGSF11) as a novel target for cancer immunotherapy of gastrointestinal and hepatocellular carcinomas. Cancer Science. 96(8) :498-506.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
43,763 Da
NCBI Official Full Name
Immunoglobulin superfamily, member 11
NCBI Official Synonym Full Names
immunoglobulin superfamily member 11
NCBI Official Symbol
IGSF11
NCBI Official Synonym Symbols
CT119; VSIG3; Igsf13; BT-IgSF; CXADRL1
NCBI Protein Information
immunoglobulin superfamily member 11
Protein Family
NCBI Description
IGSF11 is an immunoglobulin (Ig) superfamily member that is preferentially expressed in brain and testis. It shares significant homology with coxsackievirus and adenovirus receptor (CXADR; MIM 602621) and endothelial cell-selective adhesion molecule (ESAM).[supplied by OMIM, Apr 2005]
Research Articles on IGSF11
Similar Products
Product Notes
The IGSF11 (Catalog #AAA8115030) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 229aa. AAA Biotech's IGSF11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the IGSF11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly24 0. It is sometimes possible for the material contained within the vial of "IGSF11, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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