SEMA4A cell lysate

Human SEMA4A HEK293 Overexpression Lysate

Cat. Num. AAA8114766

• Gene Names: SEMA4A; RP35; SEMB; SEMAB; CORD10
• Applications: Western Blot
• Synonyms: SEMA4A; Human SEMA4A HEK293 Overexpression Lysate; Human Semaphorin 4A/SEMA4A/Semaphorin B HEK293 Cell Lysate (WB positive control); semaphorin 4A; Human CORD10 Overexpression Lysate; Human RP35 Overexpression Lysate; Human SEMAB Overexpression Lysate; Human SEMB Overexpression Lysate; SEMA4A cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 663aa
• Sequence: Met1-His683

Applicable Applications for SEMA4A cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human SEMA4A (NP_071762.2) extracellular domain (Met 1-His 683) was expressed, with a polyhistidine tag at the C-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.