Carboxypeptidase E/CPE Cell Lysate | CPE cell lysate
Human Carboxypeptidase E/CPE HEK293 Overexpression Lysate
Applications
Western Blot
Synonyms
Carboxypeptidase E/CPE; Human Carboxypeptidase E/CPE HEK293 Overexpression Lysate; Human Carboxypeptidase E/CPE HEK293 Cell Lysate (WB positive control); carboxypeptidase E; CPE cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
666aa
Sequence
Met1-Ser453
Applicable Applications for CPE cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1) (Met 1-Ser 453) was expressed with the C-terminal fused Fc region of human IgG1.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CPE cell lysate
Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.
This Human Carboxypeptidase E/CPE overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Carboxypeptidase E/CPE protein from the overexpression lysate was verified.
This Human Carboxypeptidase E/CPE overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Carboxypeptidase E/CPE protein from the overexpression lysate was verified.
References
Manser, E. et al., 1990, Biochem. J. 267: 517-525. Cool, D.R. et al., 1997, Cell. 88: 73-83. Song, L. and Fricker, L. 1995, J. Neurochem. 65: 444-453. Dhanvantari, S. et al., 2000, J. Biol. Chem. 275: 29887-29893. Jeffrey, K.D. et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105: 8452-8457
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,151 Da
NCBI Official Full Name
carboxypeptidase E preproprotein
NCBI Official Synonym Full Names
carboxypeptidase E
NCBI Official Symbol
CPE
NCBI Protein Information
carboxypeptidase E; CPH; carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase; insulin granule-associated carboxypeptidase; cobalt-stimulated chromaffin granule carboxypeptidase
UniProt Protein Name
Carboxypeptidase E
Protein Family
UniProt Gene Name
CPE
UniProt Synonym Gene Names
CPE; CPH
UniProt Entry Name
CBPE_HUMAN
NCBI Description
This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq, Jul 2008]
Uniprot Description
CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Protease; EC 3.4.17.10; Secreted
Chromosomal Location of Human Ortholog: 4q32.3
Cellular Component: Golgi apparatus; secretory granule membrane; plasma membrane; nucleus
Molecular Function: carboxypeptidase activity; zinc ion binding; neurexin binding; cell adhesion molecule binding; metallocarboxypeptidase activity
Biological Process: insulin processing; cellular protein metabolic process; metabolic process; neuropeptide signaling pathway; protein modification process; proteolysis
Research Articles on CPE
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Product Notes
The CPE cpe (Catalog #AAA8113431) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 666aa. AAA Biotech's Carboxypeptidase E/CPE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CPE cpe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser45 3. It is sometimes possible for the material contained within the vial of "Carboxypeptidase E/CPE, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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