Related Product Information for SNAP-25 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat SNAP-25 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat SNAP-25 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat SNAP-25 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat SNAP-25 can be calculated.
NCBI Description
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 20p12-p11.2
Cellular Component: cell junction; cytoplasm; cytosol; growth cone; membrane; myelin sheath; neuron projection; perinuclear region of cytoplasm; plasma membrane; presynaptic membrane; SNARE complex; trans-Golgi network; vesicle; voltage-gated potassium channel complex
Molecular Function: calcium-dependent protein binding; protein binding; SNAP receptor activity; syntaxin-1 binding
Biological Process: associative learning; energy reserve metabolic process; glutamate secretion; locomotory behavior; neurotransmitter secretion; neurotransmitter uptake; regulation of insulin secretion; synaptic transmission; synaptic vesicle docking during exocytosis; synaptic vesicle exocytosis; synaptic vesicle fusion to presynaptic membrane; synaptic vesicle priming
Disease: Myasthenic Syndrome, Congenital, 18
Product Notes
The Rat SNAP-25 snap25 (Catalog #
AAA765043) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765043 ELISA Kit recognizes Rat SNAP-25.
It is sometimes possible for the material contained within the vial of
"Synaptosome Associated Protein 25, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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