Rabbit HINT Polyclonal Antibody | anti-HINT antibody

Rabbit anti-Escherichia coli (strain K12) HINT Polyclonal Antibody

• Gene Names: hinT; ECK1089; JW1089; ycfF
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: HINT; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) HINT Polyclonal Antibody; HINT Antibody; Purine nucleoside phosphoramidase; EC 3.9.1.-; Histidine triad nucleotide binding protein HinT; HIT protein; hinT ycfF b1103 JW1089; anti-HINT antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-HINT antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) hinT protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 49176077
• NCBI GeneID: 948549
• NCBI Accession #: NP_415621.3
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0ACE7; P36950; P75945
• Molecular Weight: 13,241 Da
• NCBI Official Full Name: purine nucleoside phosphoramidase, dadA activator protein
• NCBI Official Symbol: hinT
• NCBI Official Synonym Symbols: ECK1089; JW1089; ycfF
• NCBI Protein Information: purine nucleoside phosphoramidase, dadA activator protein
• UniProt Protein Name: Purine nucleoside phosphoramidase
• UniProt Gene Name: hinT
• UniProt Synonym Gene Names: HIT protein

NCBI Description

HinT hydrolyzes lysyl-AMP, an activity that may be involved in the regulation of translation or the scavanging of aminoacyl-adenylates (Chou, 2006). hinT is essential for growth under high salt conditions (Chou, 2005). [More information is available at EcoGene: EG12172]. HinT is a purine nucleoside phosphoramidase with a preference for purine nucleoside phosphoramidates. [More information is available at EcoCyc: EG12172].

Uniprot Description

Hydrolyzes purine nucleotide phosphoramidates, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate), guanosine 5'monophosphomorpholidate (GMP-morpholidate) and tryptamine 5'guanosine monophosphate (TpGd) (PubMed:15703176, PubMed:20934431). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine--tRNA ligase, and lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) (PubMed:15703176). Is essential for the activity of the enzyme D-alanine dehydrogenase (DadA) and is required for E.coli to grow on D-alanine as a sole carbon source (PubMed:21754980). Is also required for growth at high salt concentrations (PubMed:15703176).