Dulbecco's MEM Culture Media
Dulbecco's MEM (DMEM) F-12 w/o Tryptophan
Purity
Cell Culture Grade
Synonyms
Dulbecco's MEM; Dulbecco's MEM (DMEM) F-12 w/o Tryptophan; Dulbecco's MEM culture media
Purity/Purification
Cell Culture Grade
Form/Format
Light orange, homogeneous, free flowing powder.
Endotoxin Level
< 1 EU/ml
pH
7.0
Solubility
Pink, clear, complete
Directions per Liter
Dissolve 12.048g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not auto clave. Contains heat-labile compounds that can be damaged with auto claving.
Components shown as g/liter
Inorganic Salts:
Calcium Chloride 2H2O: 0.154
Cupric Sulfate•H2O: 0.0000013
Ferric Nitrate•9H2O: 0.00005
Ferrous Sulfate•7H2O: 0.000417
Magnesium Chloride 6H2O: 0.0612
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.3118
Sodium Chloride: 6.996
Sodium Phosphate dibasic: 0.07102
Sodium Phosphate monobasic: 0.0543
Zinc Sulfate•7H2O: 0.000432
Amino Acids:
L-Alanine: 0.00445
L-Arginine•HCl: 0.1475
L-Asparagine•H2O: 0.0075
L-Aspartic Acid: 0.00665
L-Cystine•2HCl: 0.03129
L-Cysteine•HCl•H2O: 0.01756
L-Glutamic Acid : 0.00735
L-Glutamine: 0.365
Glycine: 0.01875
L-Histidine HCl•H2O: 0.03148
L-Isoleucine: 0.05447
L-Leucine: 0.05905L
L-Lysine•HCl: 0.09125
L-Methionine: 0.01724
L-Phenylalanine: 0.03548
L-Proline: 0.01725
L-Serine: 0.02625
L-Threonine: 0.05345
L-Tryptophan: Absent
L-Tyrosine•2Na•2H20: 0.05579
L-Valine: 0.05285
Vitamins:
Biotin: 0.0000035
Choline Chloride: 0.00898
Folic Acid: 0.00266
i-inositol: 0.0126
Nicotinamide: 0.00202
D-Pantothenic Acid•Ca: 0.00224
Pyridoxine HCl: 0.000031
Pyridoxal Hydrochloride: 0.002
Riboflavin: 0.000219
Thiamine HCl: 0.00217
Vitamin B12: 0.00068
Other:
D-Glucose: 3.15
Hypoxanthine(Na): 0.0021
Linoleic Acid: 0.000042
Lipoic Acid: 0.000105
Phenol Red: 0.0081
Putrescine•HCl: 0.000081
Pyruvic Acid, Sodium: 0.055
Thymidine: 0.000365
Total: 12.048g/Liter
Calcium Chloride 2H2O: 0.154
Cupric Sulfate•H2O: 0.0000013
Ferric Nitrate•9H2O: 0.00005
Ferrous Sulfate•7H2O: 0.000417
Magnesium Chloride 6H2O: 0.0612
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.3118
Sodium Chloride: 6.996
Sodium Phosphate dibasic: 0.07102
Sodium Phosphate monobasic: 0.0543
Zinc Sulfate•7H2O: 0.000432
Amino Acids:
L-Alanine: 0.00445
L-Arginine•HCl: 0.1475
L-Asparagine•H2O: 0.0075
L-Aspartic Acid: 0.00665
L-Cystine•2HCl: 0.03129
L-Cysteine•HCl•H2O: 0.01756
L-Glutamic Acid : 0.00735
L-Glutamine: 0.365
Glycine: 0.01875
L-Histidine HCl•H2O: 0.03148
L-Isoleucine: 0.05447
L-Leucine: 0.05905L
L-Lysine•HCl: 0.09125
L-Methionine: 0.01724
L-Phenylalanine: 0.03548
L-Proline: 0.01725
L-Serine: 0.02625
L-Threonine: 0.05345
L-Tryptophan: Absent
L-Tyrosine•2Na•2H20: 0.05579
L-Valine: 0.05285
Vitamins:
Biotin: 0.0000035
Choline Chloride: 0.00898
Folic Acid: 0.00266
i-inositol: 0.0126
Nicotinamide: 0.00202
D-Pantothenic Acid•Ca: 0.00224
Pyridoxine HCl: 0.000031
Pyridoxal Hydrochloride: 0.002
Riboflavin: 0.000219
Thiamine HCl: 0.00217
Vitamin B12: 0.00068
Other:
D-Glucose: 3.15
Hypoxanthine(Na): 0.0021
Linoleic Acid: 0.000042
Lipoic Acid: 0.000105
Phenol Red: 0.0081
Putrescine•HCl: 0.000081
Pyruvic Acid, Sodium: 0.055
Thymidine: 0.000365
Total: 12.048g/Liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Dulbecco's MEM culture media
Dulbecco's MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
Product Categories/Family for Dulbecco's MEM culture media
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Product Notes
The Dulbecco's MEM (Catalog #AAA653056) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Dulbecco's MEM, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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