Rabbit anti-Human UIMC1 Polyclonal Antibody | anti-UIMC1 antibody
UIMC1 (Ubiquitin Interaction Motif-containing Protein 1, BRCA1-A Complex Subunit RAP80, Receptor-associated Protein 80, RAP80, Retinoid X Receptor-interacting Protein 110, RXRIP110, X2HRIP110)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
NCBI and Uniprot Product Information
Uniprot Description
Function: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. Ref.1 Ref.10 Ref.11 Ref.16 Ref.17 Ref.18 Ref.23 Ref.25 Ref.26
Subunit structure: Interacts with TSP57
By similarity. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I. Ref.1 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.17 Ref.18 Ref.21 Ref.22 Ref.23
Subcellular location: Nucleus. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs). Ref.1 Ref.10 Ref.11 Ref.16 Ref.17 Ref.18
Tissue specificity: Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. Ref.1
Domain: The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions. Ref.24The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas. Ref.24
Post-translational modification: Sumoylated. Ref.9Phosphorylated upon DNA damage by ATM or ATR. Ref.10 Ref.16 Ref.17 Ref.18 Ref.19
Sequence similarities: Belongs to the RAP80 family.Contains 2 UIM (ubiquitin-interacting motif) repeats.
Sequence caution: The sequence AAH06078.1 differs from that shown. Reason: Erroneous termination at position 386. Translated as Glu.