Rabbit anti-Human APRT Polyclonal Antibody | anti-APRT antibody
APRT, NT (Adenine Phosphoribosyltransferase) (FITC)
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with APRT Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Western Blot (WB)
(Western blot analysis of APRT (arrow) using rabbit polyclonal APRT Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APRT gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of APRT Antibody (N-term) in A2058(lane 1),MCF-7(lane 2) cell line lysates (35ug/lane). APRT (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Protein type: EC 2.4.2.7; Transferase; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 16q24
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: adenine binding; adenine phosphoribosyltransferase activity; AMP binding
Biological Process: lactation; grooming behavior; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine salvage; purine base metabolic process
Disease: Adenine Phosphoribosyltransferase Deficiency