Rabbit anti-Human Akt1 Polyclonal Antibody | anti-AKT1 antibody

Akt1, phosphorylated (Thr 308) (v-akt Murine Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) (Preservative Free)

Cat. Num. AAA627567

• Gene Names: AKT1; AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Akt1; Polyclonal Antibody; phosphorylated (Thr 308) (v-akt Murine Thymoma Viral Oncogene Homolog 1; C-AKT; MGC99656; PKB; PRKBA; Protein kinase B; RAC; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha) (Preservative Free); Anti -Akt1; anti-AKT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Only recognizes AKT1 phosphorylated at Threonine 308, and does not crossreact with other phosphorylation sites or unphosphorylated AKT1. Species Crossreactivity: Human.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4. No preservative added.

• Applicable Applications for anti-AKT1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Immunoprecipitation and Western Blot.; Dilution: ELISA: 0.1-1.0ug/ml; Immunoprecipitation: Use 3-5ug for 10e6 cells.; Western Blot: 0.5-2.0ug/ml; Predicted molecular weight: 56kD.
• Immunogen: Synthetic peptide corresponding to AKT1 phosphorylated at Threonine 308 (Human).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-AKT1 antibody

The family of serine/threonine protein kinases Akt (also called protein kinase B or PKB) plays a key role in a variety of biological processes including cell survival, cell growth, gene expression, and oncogenesis. Upon cell stimulation, the kinase is translocated to the plasma membrane, where it is phosphorylated on two amino acids, Thr 308 in the catalytic domain and Ser 473 in the COOH-terminal regulatory domain. The activation/phosphorylation of AKT/PKB is essential in its cellular signal transduction.

Product Categories/Family for anti-AKT1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 18027298
• NCBI GeneID: 207
• UniProt Accession #: P31749; Q9BWB6; B2RAM5; Q9BV07
• Molecular Weight: 55,686 Da
• NCBI Official Full Name: AKT1
• NCBI Official Synonym Full Names: v-akt murine thymoma viral oncogene homolog 1
• NCBI Official Symbol: AKT1
• NCBI Official Synonym Symbols: AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA
• NCBI Protein Information: RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; protein kinase B; proto-oncogene c-Akt; rac protein kinase alpha
• UniProt Protein Name: RAC-alpha serine/threonine-protein kinase
• Protein Family: Potassium channel
• UniProt Gene Name: AKT1
• UniProt Synonym Gene Names: PKB; RAC
• UniProt Entry Name: AKT1_HUMAN

NCBI Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Uniprot Description

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: EC 2.7.11.1; Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; AKT family

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: microtubule cytoskeleton; nucleoplasm; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; enzyme binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK cascade; positive regulation of nitric oxide biosynthetic process; regulation of myelination; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; regulation of cell migration; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; response to heat; T cell costimulation; positive regulation of fat cell differentiation; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; regulation of nitric-oxide synthase activity; response to oxidative stress; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; positive regulation of cellular protein metabolic process; regulation of glycogen biosynthetic process; positive regulation of glycogen biosynthetic process; negative regulation of cell size; negative regulation of caspase activity; glucose transport; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; nitric oxide biosynthetic process; inflammatory response; cell differentiation; activated T cell apoptosis; aging; epidermal growth factor receptor signaling pathway; negative regulation of proteolysis; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; positive regulation of peptidyl-serine phosphorylation; endocrine pancreas development; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; cell proliferation; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of vasoconstriction; insulin receptor signaling pathway; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; hyaluronan metabolic process; phosphorylation

Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer