Rabbit IRS-2 Polyclonal Antibody | anti-IRS2 antibody

IRS-2, phosphorylated (Ser731) (Insulin Receptor Substrate 2) (Azide and BSA Free)

Cat. Num. AAA615926

• Gene Names: IRS2; IRS-2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified from rabbit serum by epitope affinity chromatography.
• Synonyms: IRS-2; Polyclonal Antibody; phosphorylated (Ser731) (Insulin Receptor Substrate 2) (Azide and BSA Free); Anti -IRS-2; anti-IRS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human IRS-2 when phosphorylated at serine 731.
• Purity/Purification: Affinity Purified; Purified from rabbit serum by epitope affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Also available with azide. See I8700-18.

• Applicable Applications for anti-IRS2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: ELISA: 0.1-1.0ug/ml; Immunoprecipation: 3-5ug/extract from 10e7 cells; Western Blot: 0.5-2.0ug/ml
• Immunogen: Synthetic phosphopeptide derived from the human IRS-2 sequence, corresponding to the serine 731 phosphorylation site. This site corresponds to serine 133 in mouse and serine 233 in rat.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-IRS2 antibody

Insulin exerts its effects by binding to the insulin receptor, a disulfide-linked heterotetrameric protein comprised of two alpha subunits and two beta subunits arranged in the following configuration: beta-alpha-alpha-beta. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc. This tyrosine phosphorylation produces docking sites for proteins bearing SH2 domains, such as PI3-K, Grb-2, Nck, and Crk. While tyrosine phosphorylation of insulin receptor substrates propagates signaling from insulin, serine and threonine phosphorylation by MAPK's, PKA, and PKC may reduce insulin signaling.

Product Categories/Family for anti-IRS2 antibody

Antibodies; Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 38683860
• NCBI GeneID: 8660
• NCBI Accession #: NP_003740.2
• NCBI GenBank Nucleotide #: NM_003749.2
• UniProt Accession #: Q96RG4; Q8TF73; Q96RG5; Q9P084; Q9UP29; Q9Y4H2
• Molecular Weight: 137,319 Da
• NCBI Official Full Name: insulin receptor substrate 2
• NCBI Official Synonym Full Names: insulin receptor substrate 2
• NCBI Official Symbol: IRS2
• NCBI Official Synonym Symbols: IRS-2
• NCBI Protein Information: insulin receptor substrate 2; OTTHUMP00000018696
• UniProt Protein Name: Insulin receptor substrate 2 insertion mutant
• Protein Family: Insulin receptor substrate
• UniProt Gene Name: IRS2
• UniProt Entry Name: Q96RG4_HUMAN

NCBI Description

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq]

Uniprot Description

IRS2: May mediate the control of various cellular processes by insulin. Interacts with PHIP.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; phosphoinositide 3-kinase binding; protein phosphatase binding; protein kinase binding; insulin receptor binding

Biological Process: negative regulation of kinase activity; regulation of lipid metabolic process; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; lipid homeostasis; signal transduction; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; positive regulation of B cell proliferation; response to glucose stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of insulin secretion; glucose metabolic process; positive regulation of fatty acid beta-oxidation; cell proliferation; cellular response to insulin stimulus; insulin receptor signaling pathway; innate immune response; brain development; negative regulation of B cell apoptosis; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent