Rabbit anti-Human EP4 Receptor Polyclonal Antibody | anti-PTGER4 antibody

EP4 Receptor

Cat. Num. AAA611305

• Gene Names: PTGER4; ep4
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: EP4 Receptor; Polyclonal Antibody; Anti -EP4 Receptor; anti-PTGER4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EP4 Receptor. No significant homology exist with other EP (1, 2 or 3) Receptors. Species sequence homology
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.

• Applicable Applications for anti-PTGER4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 0.5-1ug/ml
• Immunogen: A 16 AA peptide within 2nd extracellular domain of Human EP4 (1) coupled to KLH. An antibody made to this epitope has been shown to recognize 47 and 78kD EP4 bands in the kidney (2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PTGER4 antibody

Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid) and include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2. The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli by the action of phospholipase A2 and are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases. Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation. A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release, and renal salt and water transport have been described. PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4 that are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes. In general, EP receptors display a protein topology typical of GPCR-7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus.

EP4 (mouse 513 aa, rat 488 aa; human 488 aa, chromosome 5p13.1, ~ 90% interspecies homology) is expressed in intestine, lung, thymus, kidney, uterus, pancreas, spleen, heart, stomach, and brain. EP4 knockout mice show patent ductus arteriosus, and decreased inflammatory bone resorption. EP4 activity is mediated by Gs that stimulate adenyl cyclase.It has a relaxing effect on smooth muscle and implicated in renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine functions. It was originally reported as EP2 subtype.

Product Categories/Family for anti-PTGER4 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 22536174
• NCBI GeneID: 282331
• UniProt Accession #: Q8MJ08; Q1JPJ5; Q95M50
• Molecular Weight: 53,298 Da
• NCBI Official Full Name: EP4 receptor
• NCBI Official Symbol: PTGER4
• NCBI Official Synonym Symbols: ep4
• NCBI Protein Information: prostaglandin E2 receptor EP4 subtype; prostanoid EP4 receptor; PGE receptor EP4 subtype; PGE receptor, EP4 subtype; PGE2 receptor EP4 subtype
• UniProt Protein Name: Prostaglandin E2 receptor EP4 subtype
• Protein Family: Prostaglandin E2 receptor
• UniProt Gene Name: PTGER4
• UniProt Entry Name: PE2R4_BOVIN

Uniprot Description

Function: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function

Subunit structure: Interacts with FEM1A

Subcellular location: Cell membrane; Multi-pass membrane protein

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.