Rabbit C2 Polyclonal Antibody

Rabbit anti Human C2

Cat. Num. AAA573258

• Reactivity: The antiSerum does not cross-react with any other Human plasma proteins as tested in geldiffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently sh
• Applications: Immunoprecipitation
• Synonyms: C2; Polyclonal Antibody; Rabbit anti Human C2; anti-C2 antibody

• Host: Rabbit
• Reactivity: The antiSerum does not cross-react with any other Human plasma proteins as tested in geldiffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently sh
• Clonality: Polyclonal
• Specificity: Precipitating polyclonal Rabbit antiSerum to Human C2 component of complement.
• Form/Format: Delipidated, heat inactivated, lyophilized, stable IgG fraction. No preservative added. The IgG concentration is 4 mg/ml in PBS-EDTA. No foreign proteins added.

• Applicable Applications for anti-C2 antibody: Immunoprecipitation (IP)
• Application Notes: Precipitation assays. In immunoelectrophoresis in agarose-plates use 2 ?l human plasma or equivalent against 120 ?l antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ?l antiserum in 3 mm diameter center well and 2 ?l plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
• Source: The C2 component of human complement is a single-chain polypeptide with a molecular weight of 110,000. It is present in plasma in an average concentration of 25 ?g/ml. The activated form of C2 combines with the activated C4 to form a complex with C3-convertase activity in the classical pathway of complement activation. C2 is isolated as a homogenous protein for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Product: Delipidated, heat inactivated, lyophilized, stable IgG fraction. No preservative added. The IgG concentration is 4 mg/ml in PBS-EDTA. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
• Preparation and Storage: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Related Product Information for anti-C2 antibody

The reactivity of the antiserum is restricted to native and activated C2. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of C2 or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

Product Categories/Family for anti-C2 antibody

Complement; Coagulation; Haematology