Protein A Biochemical
Protein A
Purity
>95% by SDS-PAGE analyses
Synonyms
Protein A; Protein A biochemical
Specificity
The interaction between Protein A and IgG is not equivalent for all species.Even within a species, Protein A interacts with some subclasses of IgG and not others. For instance, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1.
Purity/Purification
>95% by SDS-PAGE analyses
Form/Format
Lyophilized with no additive.
Application Notes
The Protein A molecule contains four high-affinity (Ka =108/mole) binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit (Table 1). Optimal binding occurs a pH 8.2, although binding is also good at neutral or physiological conditions (pH 7.0-7.6).
Source
Escherichia coli
Molecular Weight
Protein A is a cell wall component produced by several strains of Staphylococcusaureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series. It migrates with an apparent molecular mass of 45kDa in SDS-PAGE.
A280 of 0.1% solution
~0.165
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Endotoxin
Less than 0.1EU/ug of Protein A as determined by LAL method.
Reconstitution
Dissolve in distilled water or saline.
Preparation and Storage
2 years at -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
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Product Notes
The Protein A (Catalog #AAA546219) is a Biochemical and is intended for research purposes only. The product is available for immediate purchase. The Protein A molecule contains four high-affinity (Ka =108/mole) binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit (Table 1). Optimal binding occurs a pH 8.2, although binding is also good at neutral or physiological conditions (pH 7.0-7.6). Researchers should empirically determine the suitability of the Protein A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein A, Biochemical" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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