phospho-Paxillin (Ser-83) Peptide | Pxn peptide
phospho-Paxillin (Ser-83) Peptide
Gene Names
Pxn; Pxn
Synonyms
phospho-Paxillin (Ser-83) Peptide; Pxn peptide
Specificity
The peptide is specifically recognized by anti-Paxillin (Ser-83) phospho-specific antibody (PP1341) in ELISA, and has been shown to block the reactivity of PP1341 during Western blot. In addition, the peptide is recommended for use in blocking PP1341 reactivity in immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: Phospho-Paxillin (Ser-83) synthetic peptide corresponds to amino acid residues around serine 83 of mouse paxillin. This sequence is highly conserved in rat and human paxillin and is also found in all isoforms (alpha, beta, gamma) of paxillin.
Sequence Length
586
Application Notes
Blocking: 1:1,000
ELISA: 50 ng/well
ELISA: 50 ng/well
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Related Product Information for Pxn peptide
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPaseactivating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both ERK and p38MAPK kinases phosphorylate serine 83 in vitro. HGF stimulation of murine epithelial cells leads to ERK-mediated phosphorylation of Ser-83, which is required for HGF-induced cell spreading and migration. In addition, Ser-83 is phosphorylated in response to NGF in PC12 cells, and this phosphorylation may be involved in neurite extension.
References
• Huang, C. et al. (2004) J Cell Biol. 164(4):593-602.
• Ishibe, S. et al. (2004) Mol. Cell 16 :257-267.
• Ishibe, S. et al. (2004) Mol. Cell 16 :257-267.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63888 Da
NCBI Official Full Name
paxillin
NCBI Official Synonym Full Names
paxillin
NCBI Official Symbol
Pxn
NCBI Official Synonym Symbols
Pxn
NCBI Protein Information
paxillin; myocardial ischemic preconditioning associated protein 7
UniProt Protein Name
Paxillin
Protein Family
UniProt Gene Name
Pxn
UniProt Entry Name
PAXI_RAT
Uniprot Description
Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion)
Subunit structure: Binds to vinculin, to the SH3 domain of c-SRC and to focal adhesion kinase. Interacts with GIT1 and NUDT16L1/SDOS. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2 and PDCD10
By similarity. Interacts with PARVA and TGFB1I1. Ref.2 Ref.3
Subcellular location: Cytoplasm › cytoskeleton
By similarity. Cell junction › focal adhesion
Post-translational modification: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens
By similarity. Ref.4
Sequence similarities: Belongs to the paxillin family.Contains 4 LIM zinc-binding domains.
Research Articles on Pxn
Similar Products
Product Notes
The Pxn pxn (Catalog #AAA474050) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Blocking: 1:1,000 ELISA: 50 ng/well. Researchers should empirically determine the suitability of the Pxn pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Phospho-Pa xillin (Ser-83) synthetic peptide correspond s to amino acid residues around serine 83 of mouse paxillin. This sequence is highly conserved in rat and human paxillin and is also found in all isoforms (alpha, beta, gamma) of paxillin. It is sometimes possible for the material contained within the vial of "phospho-Paxillin (Ser-83) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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