Rabbit anti-Human Kappa Light Chain Polyclonal Antibody | anti-Igk antibody

Rabbit anti Kappa Light Chain (B-Cell Marker) Polyclonal Antibody

Cat. Num. AAA460125

• Gene Names: Igk; kappa
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Kappa Light Chain; Polyclonal Antibody; Rabbit anti Kappa Light Chain (B-Cell Marker) Polyclonal Antibody; Immunoglobulin kappa chain; anti-Igk antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 219

• Applicable Applications for anti-Igk antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 416539
• NCBI GeneID: 243469
• UniProt Accession #: A2P1G9
• Molecular Weight: 24,086 Da
• NCBI Official Full Name: kappa light chain, partial
• NCBI Official Synonym Full Names: immunoglobulin kappa chain complex
• NCBI Official Symbol: Igk
• NCBI Official Synonym Symbols: kappa
• NCBI Protein Information: immunoglobulin kappa chain complex; kappa light chain variable region
• UniProt Protein Name: Kappa light chain
• UniProt Gene Name: Igkc

NCBI Description

Summary:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the kappa light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments in this cluster are incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq, Jul 2008]