Mouse anti-Human Melanoma Antibody
Melanoma, HMB45
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Melanoma; HMB45; anti-Melanoma antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
HMB45
Form/Format
Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.
Applicable Applications for anti-Melanoma antibody
Immunohistochemistry (IHC) Paraffin
Application Notes
Working Concentration: (RTU) neat
Immunogen
Extract from lymph node metastases of melanoma
Positive Control
Melanoma
Incubation Time
60 min at RT
Pre-Treatment
Pre-treatment with pronase 0,1% or unmasking fluid G ()
Pre-Treatment
Pre-treatment with pronase 0,1% or unmasking fluid G ()
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for anti-Melanoma antibody
The antibody HMB45 is used for the discrimination of Melanoma from normal Melanocytes. Melanoic Schwannoma Clear Cell Sarcoma will also be stained. Negative are tumours of epithelial, lymphoid, glial and mesenchymal origin. Melanocytes are pigmented, melanin containing cells being located mainly in the epidermis. They protect tissues from UVB irradiation of the sun. The antibody HMB45 reacts with fetal and neonatal Melanocytes, with junctional and blue naevus cells, but not with intradermal naevi and adult melanocytes. Neuraminidase-sensitive glycoconjugate on the surface of human melanosomes.
References
1. Gown A.M., et al., (1986) Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am. J. Pathol. 123; 195 ff. 2. Kapur R.P., et al. (1992) Anti-melanoma monoclonal antibody HMB45 identified an oncofetal glycoconjugate associated with immature melanosomes. J. Histochem Cytochem 40(2); 207 ff. 3. Leong A.S.-Y., and Millos J. (1989) An assessment of a melanoma-specific antibody HMB-45 and other immunohistochemical markers of malignant melanoma in paraffin-embedded tissues. Surg. Pathol. 2; 137 ff.
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Product Notes
The Melanoma (Catalog #AAA397283) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Melanoma, HMB45 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Melanoma can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin. Working Concentration: (RTU) neat. Researchers should empirically determine the suitability of the Melanoma for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Melanoma, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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