Mouse anti-Human Melan-A/MART-1 Antibody
Melan-A/MART-1
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Melan-A/MART-1; anti-Melan-A/MART-1 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
A103
Form/Format
Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.
Applicable Applications for anti-Melan-A/MART-1 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes
Dilution: 1:10-1:20
Immunogen
Recombinant Melan-A protein
Positive Control
Melanoma
Incubation Time
60 min at RT
Pre-Treatment
Heat pre-treatment of formalin fixed material with Unmasking Fluid G is recommended.
Pre-Treatment
Heat pre-treatment of formalin fixed material with Unmasking Fluid G is recommended.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for anti-Melan-A/MART-1 antibody
The antibody A103 is suitable for the detection of Melan-A antigen (syn. MART-1) in cells of melanoic origin. The antibody will label 80-90% of metastasing malign melanoma (1, 6). Melan-A-negative melanoma usually are of the spindle cell or desmoblastic type. The antibody also reacts positive with angiomyolipoma (4) and steroid hormone producing cells of the adrenal gland, ovary, testis and especially with carcinoma of these organs. Melan-A is a melanoma specific antigen, which is detected by cytotoxic T-cells. It is exprimed by benign naevi and melanoma cells. 20-22 kDA Melan-A protein, no cross-reactivity to MAGE-1 and Tyrosinase.
References
1. Jungbluth AA, Busam KJ, Gerald WL, Stockert E, Coplan KA, Iversen K, MacGregor DP, Old LJ, Chen YT. A103: An anti-melan-a monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissues. Am J Surg Pathol. 1998 May;22(5):595-602. 2.Berset M, Cerottini JP, Guggisberg D, Romero P, Burri F, Rimoldi D, Panizzon RG. Expression of Melan-A/MART-1 antigen as a prognostic factor in primary cutaneous melanoma. Int J Cancer. 2001 Jan 20;95(1):73-7. 3. Florell SR, Zone JJ, Gerwels JW. Basal cell carcinomas are populated by melanocytes and Langerhan's cells. Am J Dermatopathol. 2001 Feb;23(1):24-8. 4. Zavala-Pompa A, Folpe AL, Jimenez RE, Lim SD, Cohen C, Eble JN, Amin MB. Immunohistochemical study of microphthalmia transcription factor and tyrosinase in angiomyolipoma of the kidney, renal cell carcinoma, and renal and retroperitoneal sarcomas: comparative evaluation with traditional diagnostic markers. Am J Surg Pathol. 2001 Jan;25(1):65-70. 5. Schmuth M, Spotl L, Zelger B, Weinlich G, Zelger B. Clear cells in acral melanoma. Eur J Dermatol. 2001 Jan-Feb;11(1):21-4.
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Product Notes
The Melan-A/MART-1 (Catalog #AAA397282) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Melan-A/MART-1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Melan-A/MART-1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin. Dilution: 1:10-1:20. Researchers should empirically determine the suitability of the Melan-A/MART-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Melan-A/MART-1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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