Rabbit anti-Human Ceramide Kinase Polyclonal Antibody | anti-CERK antibody

Ceramide Kinase

Cat. Num. AAA395700

• Gene Names: CERK; LK4; hCERK; dA59H18.2; dA59H18.3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Ammonium Sulfate Precipitation
• Synonyms: Ceramide Kinase; Polyclonal Antibody; Ceramide kinase; Acylsphingosine kinase; hCERK; Lipid kinase 4; LK4; ; anti-CERK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 537

• Applicable Applications for anti-CERK antibody: Western Blot (WB), ELISA (EIA)
• Immunogen: Synthetic peptide derived from human ceramide kinase protein
• Positive Control: High level expression in heart, brain, skeletal muscle, kidney and liver
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-CERK antibody

Sphingolipids, in addition to being structural components of membranes, regulate cell-cell and cell-substrate interactions, proliferation, and differentiation. Members of this diverse group of lipids have emerged as a novel class of signaling molecules that also regulate phagocytosis. The mechanisms by which sphingolipids exert these effects remain incompletely defined. More than a decade ago, it was found that ceramide can be phosphorylated to ceramide 1-phosphate (C1P). Ceramide kinase (CERK) and its phosphorylated product ceramide 1-phosphate (C1P) are central players in inflammation and cancer. The product of CERK activity, ceramide 1-phosphate (C1P), has been reported to have mitogenic effects. C1P is a direct activator of cytosolic phospholipase A2 and is involved in arachidonic acid release. CERK is a mediator of Ca2+-dependent degranulation in mast cells. In both arachidonic acid release and mast cell degranulation, the intracellular elevation of Ca2+ is a central event that acts as a regulatory mechanism of CERK activity. C1P is found in brain synaptic vesicles, and plays a role in regulating the secretion of neurotransmitters. CERK activity exists in HL-60 cells where the C1P is derived from ceramide released from sphingomyelin. The expressed kinase has specific ceramide phosphorylating activity. CERKs exist in a variety of cellular organisms, including plants, nematodes, insects, and vertebrates.

Product Categories/Family for anti-CERK antibody

Polyclonal Antibody; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 20336726
• NCBI GeneID: 64781
• NCBI Accession #: NP_073603.2
• NCBI GenBank Nucleotide #: NM_022766.5
• UniProt Accession #: Q8TCT0; Q9BYB3; Q9UGE5; A0JNT4; A8K611
• Molecular Weight: 59977
• NCBI Official Full Name: ceramide kinase
• NCBI Official Synonym Full Names: ceramide kinase
• NCBI Official Symbol: CERK
• NCBI Official Synonym Symbols: LK4; hCERK; dA59H18.2; dA59H18.3
• NCBI Protein Information: ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase
• UniProt Protein Name: Ceramide kinase
• Protein Family: Ceramide kinase
• UniProt Gene Name: CERK
• UniProt Synonym Gene Names: KIAA1646; hCERK; LK4
• UniProt Entry Name: CERK1_HUMAN

NCBI Description

CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. Ref.8

Catalytic activity: ATP + ceramide = ADP + ceramide 1-phosphate.

Cofactor: Calcium.Magnesium.

Enzyme regulation: Inhibited by sulfatide. Ref.8

Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein Ref.8.

Tissue specificity: High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.

Sequence similarities: Contains 1 DAGKc domain.

Biophysicochemical propertiespH dependence:Optimum pH is 6.0-7.5.