Mouse Zinc finger protein Aiolos (IKZF3) ELISA Kit | IKZF3 elisa kit

Mouse Zinc finger protein Aiolos (IKZF3) ELISA Kit

Cat. Num. AAA287593

• Gene Names: Ikzf3; Aiolos; Zfpn1a3; Znfn1a3; 5830411O07Rik
• Reactivity: Mouse
• Synonyms: Zinc finger protein Aiolos (IKZF3); Mouse Zinc finger protein Aiolos (IKZF3) ELISA Kit; AIO; AIOLOS; ZNFN1A3; aiolos; zinc finger protein; subfamily 1A; 3 (Aiolos); IKZF3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse IKZF3. No significant cross-reactivity or interference between Mouse IKZF3 and analogues was observed.
• Sequence Length: 507

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for IKZF3 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate IKZF3 in samples. An antibody specific for IKZF3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IKZF3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IKZF3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IKZF3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: IKZF3 encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling.
Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 113374152
• NCBI GeneID: 22780
• NCBI Accession #: NP_035901.1
• NCBI GenBank Nucleotide #: NM_011771.1
• UniProt Accession #: O08900; B1AQE6
• Molecular Weight: 57,967 Da
• NCBI Official Full Name: zinc finger protein Aiolos
• NCBI Official Synonym Full Names: IKAROS family zinc finger 3
• NCBI Official Symbol: Ikzf3
• NCBI Official Synonym Symbols: Aiolos; Zfpn1a3; Znfn1a3; 5830411O07Rik
• NCBI Protein Information: zinc finger protein Aiolos
• UniProt Protein Name: Zinc finger protein Aiolos
• Protein Family: Zinc finger protein
• UniProt Gene Name: Ikzf3
• UniProt Synonym Gene Names: Zfpn1a3; Znfn1a3

Uniprot Description

Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Binds to GGGAA. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.