Mouse Deoxynucleotidyltransferase terminal-interacting protein 2 ELISA Kit | DNTTIP2 elisa kit
Mouse Deoxynucleotidyltransferase terminal-interacting protein 2 (DNTTIP2) ELISA Kit
Gene Names
Dnttip2; AA408582; AU014960; HSU15552; 4930588M11Rik
Reactivity
Mouse
Synonyms
Deoxynucleotidyltransferase terminal-interacting protein 2; Mouse Deoxynucleotidyltransferase terminal-interacting protein 2 (DNTTIP2) ELISA Kit; ERBP; FCF2; HSU15552; LPTS-RP2; MGC163494; RP4-561L24.1; TdIF2; acidic 82 kDa protein mRNA (HSU15552)|estrogen receptor binding protein; DNTTIP2 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse DNTTIP2. No significant cross-reactivity or interference between Mouse DNTTIP2 and analogues was observed.
Sequence Length
756
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DNTTIP2 elisa kit
ERBP C-terminal and N-terminal fragments interacted with the DNA binding domain and hinge region of ER-alpha. Binding of the C-terminal ERBP fragment was enhanced in the presence of estrogen. ERBP also bound ER-beta (ESR2), but this interaction was not affected by estrogen. Overexpression of ERBP enhanced ER-alpha-mediated transactivation in an estrogen-dependent manner. Transcriptional activity assay showed that ERBP itself lacked intrinsic transcription activity.
The deduced 756-amino acid protein has a calculated molecular mass of approximately 82 kD. ERBP localized to the nucleus in HeLa cells and in transfected COS-7 cells. Northern blot analysis detected expression of a 2.4-kb transcript in all tissues examined, with high levels in testis.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DNTTIP2 in samples. An antibody specific for DNTTIP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNTTIP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNTTIP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNTTIP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The deduced 756-amino acid protein has a calculated molecular mass of approximately 82 kD. ERBP localized to the nucleus in HeLa cells and in transfected COS-7 cells. Northern blot analysis detected expression of a 2.4-kb transcript in all tissues examined, with high levels in testis.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DNTTIP2 in samples. An antibody specific for DNTTIP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNTTIP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNTTIP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNTTIP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,277 Da
NCBI Official Full Name
deoxynucleotidyltransferase terminal-interacting protein 2
NCBI Official Synonym Full Names
deoxynucleotidyltransferase, terminal, interacting protein 2
NCBI Official Symbol
Dnttip2
NCBI Official Synonym Symbols
AA408582; AU014960; HSU15552; 4930588M11Rik
NCBI Protein Information
deoxynucleotidyltransferase terminal-interacting protein 2
UniProt Protein Name
Deoxynucleotidyltransferase terminal-interacting protein 2
Protein Family
UniProt Gene Name
Dnttip2
Uniprot Description
ERBP: Regulates the transcriptional activity of DNTT and ESR1. May function as a chromatin remodeling protein.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 3|3 G1
Cellular Component: nucleolus; nucleus
Molecular Function: RNA binding
Biological Process: regulation of transcription, DNA-templated; transcription, DNA-dependent
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Product Notes
The Mouse DNTTIP2 dnttip2 (Catalog #AAA285702) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285702 ELISA Kit recognizes Mouse DNTTIP2. It is sometimes possible for the material contained within the vial of "Deoxynucleotidyltransferase terminal-interacting protein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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