Sheep Prostaglandin G/H synthase 1 ELISA Kit | PTGS1 elisa kit

Sheep Prostaglandin G/H synthase 1 (PTGS1) ELISA Kit

Cat. Num. AAA285400

• Gene Names: PTGS1; COX1; COX-1; PGHS-1
• Reactivity: Sheep
• Synonyms: Prostaglandin G/H synthase 1; Sheep Prostaglandin G/H synthase 1 (PTGS1) ELISA Kit; RP11-542K23.6; COX1; COX3; PCOX1; PGG/HS; PGHS-1; PGHS1; PHS1; PTGHS; prostaglandin G/H synthase and cyclooxygenase|prostaglandin-endoperoxide synthase 1; PTGS1 elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep PTGS1. No significant cross-reactivity or interference between Sheep PTGS1 and analogues was observed.
• Sequence Length: 599

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PTGS1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PTGS1 in samples. An antibody specific for PTGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 57164169
• NCBI GeneID: 443551
• NCBI Accession #: NP_001009476.1
• NCBI GenBank Nucleotide #: NM_001009476.1
• UniProt Accession #: P05979
• Molecular Weight: 68,861 Da
• NCBI Official Full Name: prostaglandin G/H synthase 1
• NCBI Official Symbol: PTGS1
• NCBI Official Synonym Symbols: COX1; COX-1; PGHS-1
• NCBI Protein Information: prostaglandin G/H synthase 1
• UniProt Protein Name: Prostaglandin G/H synthase 1
• Protein Family: Prostaglandin G/H synthase
• UniProt Gene Name: PTGS1
• UniProt Synonym Gene Names: COX1; COX-1; PGH synthase 1; PGHS-1; PHS 1

NCBI Description

This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. [provided by RefSeq, Jan 2014]

Uniprot Description

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.