Sheep Dynein light chain Tctex-type 3 ELISA Kit | DYNLT3 elisa kit

Sheep Dynein light chain Tctex-type 3 (DYNLT3) ELISA Kit

Cat. Num. AAA284229

• Gene Names: DYNLT3; TCTE1L
• Reactivity: Sheep
• Synonyms: Dynein light chain Tctex-type 3; Sheep Dynein light chain Tctex-type 3 (DYNLT3) ELISA Kit; RP3; TCTE1L; TCTEX1L; t-complex-associated-testis-expressed 1-like; DYNLT3 elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep DYNLT3. No significant cross-reactivity or interference between Sheep DYNLT3 and analogues was observed.
• Sequence Length: 116

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DYNLT3 elisa kit

DYNLT3 is a member of the dynein motor protein family. DYNLT3 binds to BUB3, a spindle checkpoint protein is present on kinetochores at prometaphase. DYNLT3 can also function as a transcription regulator of Bcl-2 gene through binding to SATB1 in a dynein-independent manner.The TCTE1L cDNA encodes a deduced 116-amino acid protein that shares 50.4% sequence identity with the mouse homolog, a member of the mouse t complex, which maps to chromosome 17. Genes in the mouse t complex are involved in sperm or male germ cell differentiation and are associated with male sterility and transmission ratio distortion. Northern blot analysis detected expression of a TCTE1L 2.1-kb mRNA in fetal and adult retina, as well as in fetal brain and adult frontal cortex, lung, heart, esophagus, testis, bone marrow, and fibroblasts..

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DYNLT3 in samples. An antibody specific for DYNLT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDYNLT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYNLT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYNLT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 57526279
• NCBI GeneID: 443256
• NCBI Accession #: NP_001009762.1
• NCBI GenBank Nucleotide #: NM_001009762.1
• UniProt Accession #: Q6XXL8
• Molecular Weight: 13,037 Da
• NCBI Official Full Name: dynein light chain Tctex-type 3
• NCBI Official Symbol: DYNLT3
• NCBI Official Synonym Symbols: TCTE1L
• NCBI Protein Information: dynein light chain Tctex-type 3
• UniProt Protein Name: Dynein light chain Tctex-type 3
• Protein Family: Dynein
• UniProt Gene Name: DYNLT3
• UniProt Synonym Gene Names: TCTE1L

Uniprot Description

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Probably binds BUB3 as part of transport cargo. Required for the efficient progression through mitosis ().