Cynomolgus Monkey Glucagon like peptide 1 ELISA Kit | GLP1 elisa kit
Cynomolgus Monkey Glucagon like peptide 1 (GLP1) ELISA Kit
Reactivity
Cynomolgus Monkey
Synonyms
Cynomolgus Monkey Glucagon like peptide 1; Cynomolgus Monkey Glucagon like peptide 1 (GLP1) ELISA Kit; GLP1 elisa kit
Reactivity
Cynomolgus Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of Cynomolgus Monkey GLP1. No significant cross-reactivity or interference between Cynomolgus Monkey GLP1 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GLP1 elisa kit
Glucagon Like Peptide-1 (GLP-1) is a peptide hormone from the intestinal mucosa, which is produced from its precursor, proglucagon by post transnational processing. The mammalian proglucagon is synthesized in the neuroendocrine L-cell of the intestine and the alpha-cells of the pancreas. It contains within its structure the sequences of glucagon and two glucagon-like peptides (GLP-1 and GLP-2) in tandem flanked at their amino and carboxyl termini by dibasic residues. GLP-1 is a 37 amino acids peptide and produced in the small intestine and in the pancreas in the rat, in either C-terminal-amidated on glycine-extended form. GLP1 (7-36) amide and its receptor are present in several brain regions and may play a role in the physiological control of feeding. Several reports have been presented as follows as to the biological activities of GLP-1. GLP-1 (7-37) and (7-36) amide is known as one of the most potent insulin secretagogues.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GLP1 in samples. An antibody specific for GLP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGLP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GLP1 in samples. An antibody specific for GLP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGLP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Product Notes
The Cynomolgus Monkey GLP1 (Catalog #AAA282864) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282864 ELISA Kit recognizes Cynomolgus Monkey GLP1. It is sometimes possible for the material contained within the vial of "Cynomolgus Monkey Glucagon like peptide 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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