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Bovine 39S ribosomal protein L1 ELISA Kit | MRPL1 elisa kit

Bovine 39S ribosomal protein L1, mitochondrial (MRPL1) ELISA Kit

Gene Names
MRPL1; L1mt; MRP-L1
Reactivity
Bovine
Synonyms
39S ribosomal protein L1; Bovine 39S ribosomal protein L1; mitochondrial (MRPL1) ELISA Kit; MRPL1 elisa kit
Ordering
For Research Use Only!
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine MRPL1. No significant cross-reactivity or interference between Bovine MRPL1 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MRPL1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MRPL1 in samples. An antibody specific for MRPL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,657 Da
NCBI Official Full Name
39S ribosomal protein L1, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L1
NCBI Official Symbol
MRPL1
NCBI Official Synonym Symbols
L1mt; MRP-L1
NCBI Protein Information
39S ribosomal protein L1, mitochondrial
UniProt Protein Name
39S ribosomal protein L1, mitochondrial
Protein Family
UniProt Gene Name
MRPL1
UniProt Synonym Gene Names
L1mt; MRP-L1

Uniprot Description

MRPL1: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L1 ribosomal protein family. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; RNA-binding

Cellular Component: mitochondrial inner membrane

Molecular Function: structural constituent of ribosome

Biological Process: cleavage involved in rRNA processing; maturation of LSU-rRNA; rRNA pseudouridine synthesis; snRNA pseudouridine synthesis; translation

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Product Notes

The Bovine MRPL1 mrpl1 (Catalog #AAA282838) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282838 ELISA Kit recognizes Bovine MRPL1. It is sometimes possible for the material contained within the vial of "39S ribosomal protein L1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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