Mouse Zinc finger MYM-type protein 2 ELISA Kit | ZMYM2 elisa kit
Mouse Zinc finger MYM-type protein 2 (ZMYM2) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
The novel sequence that was fused to FGFR1, comprising the 5-prime end of the fusion transcript, was mapped to chromosome 13 with the CEPH mega-YAC library and the Genebridge 4 radiation hybrid panel. The findings were consistent with the pericentromeric 13q11-q12 assignments for the t(8;13) cytogenetic breakpoints.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZMYM2 in samples. An antibody specific for ZMYM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZMYM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMYM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMYM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that contains nine MYM-type zinc finger motifs. Expression of this gene may mediate the inhibition of hematopoietic cell development during ontogeny, and the encoded protein may also play a role in transforming growth factor-beta signaling as a Smad binding protein. [provided by RefSeq, Feb 2011]
Uniprot Description
ZNF198: a probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. The translocation between FGFR and ZNF198 (8;13)(p11;q12) is oncogenic, causing stem cell leukemia lymphoma syndrome (SCLL), a myeloproliferative disorder (MPD) that evolves to acute myeloid leukemia and/or lymphoma. The transforming activity of ZNF198-FGFR1 requires the proline-rich domains, but not the ZNF domains, of ZNF198.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 14|14 C3
Cellular Component: cytoplasm; nucleoplasm; nucleus; PML body
Molecular Function: DNA binding; metal ion binding; protein binding; ubiquitin conjugating enzyme binding; zinc ion binding
Biological Process: cytoskeleton organization; multicellular organism development; regulation of cell morphogenesis; regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on ZMYM2
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Product Notes
The Mouse ZMYM2 zmym2 (Catalog #AAA282809) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282809 ELISA Kit recognizes Mouse ZMYM2. It is sometimes possible for the material contained within the vial of "Zinc finger MYM-type protein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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