Human Polycomb protein SUZ12 ELISA Kit | SUZ12 elisa kit

Human Polycomb protein SUZ12 (SUZ12) ELISA Kit

Cat. Num. AAA281952

• Gene Names: SUZ12; CHET9; JJAZ1
• Reactivity: Human
• Synonyms: Polycomb protein SUZ12; Human Polycomb protein SUZ12 (SUZ12) ELISA Kit; SUZ12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human SUZ12. No significant cross-reactivity or interference between Human SUZ12 and analogues was observed.
• Sequence Length: 739

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for SUZ12 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SUZ12 in samples. An antibody specific for SUZ12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySUZ12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SUZ12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SUZ12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 197333809
• NCBI GeneID: 23512
• NCBI Accession #: NP_056170.2
• NCBI GenBank Nucleotide #: NM_015355.3
• UniProt Accession #: Q15022; Q96BD9
• Molecular Weight: 83,055 Da
• NCBI Official Full Name: polycomb protein SUZ12 isoform 1
• NCBI Official Synonym Full Names: SUZ12 polycomb repressive complex 2 subunit
• NCBI Official Symbol: SUZ12
• NCBI Official Synonym Symbols: CHET9; JJAZ1
• NCBI Protein Information: polycomb protein SUZ12
• UniProt Protein Name: Polycomb protein SUZ12
• Protein Family: Polycomb protein
• UniProt Gene Name: SUZ12
• UniProt Synonym Gene Names: CHET9; JJAZ1; KIAA0160; ChET 9 protein

NCBI Description

This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009]

Uniprot Description

SUZ12: a zinc finger protein. A breakpoint translocation of the cognate gene with JAZF1 has been reported in endometrial stromal sarcoma.

Protein type: C2H2-type zinc finger protein; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: ESC/E(Z) complex; nuclear body; nucleolus; nucleoplasm; nucleus; RSC-type complex; sex chromatin

Molecular Function: chromatin DNA binding; histone methyltransferase activity; histone methyltransferase activity (H3-K27 specific); metal ion binding; methylated histone residue binding; promoter-specific chromatin binding; protein binding; RNA binding

Biological Process: histone ubiquitination; negative regulation of cell differentiation; negative regulation of G0 to G1 transition; negative regulation of gene expression, epigenetic; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; regulation of transcription, DNA-templated; transcription, DNA-dependent