Mouse Transcription factor RelB (RELB) ELISA Kit | RELB elisa kit

Mouse Transcription factor RelB (RELB) ELISA Kit

Cat. Num. AAA2802069

• Gene Names: Relb; shep
• Reactivity: Mouse
• Synonyms: Transcription factor RelB (RELB); Mouse Transcription factor RelB (RELB) ELISA Kit; I-REL; IREL; REL-B; reticuloendotheliosis viral oncogene homolog B|v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)|v; RELB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse RELB. No significant cross-reactivity or interference between Mouse RELB and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate RELB in samples. An antibody specific for RELB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELB bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

Related Product Information for RELB elisa kit

Bours et al. (1994) demonstrated that human RELB, when generated in mammalian cells, formed kappa-B-binding heterodimeric complexes with p50 (NFKB1) or p52 (NFKB2). Homodimeric complexes of RELB did not show DNA-binding activity.NF-kappa-B-inducing kinase (NIK, or MAP3K14) is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al. (2008) found that overexpression of Relb, but not Rela, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classical NF-kappa-B pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using Relb -/- mice, they showed that Relb itself was required for Rankl-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo

NCBI and Uniprot Product Information

• NCBI GI #: 594190858
• NCBI GeneID: 19698
• NCBI Accession #: NP_001277386.1
• NCBI GenBank Nucleotide #: NM_001290457.1
• UniProt Accession #: Q04863; Q8VE46
• Molecular Weight: 60,305 Da
• NCBI Official Full Name: transcription factor RelB isoform 2
• NCBI Official Synonym Full Names: avian reticuloendotheliosis viral (v-rel) oncogene related B
• NCBI Official Symbol: Relb
• NCBI Official Synonym Symbols: shep
• NCBI Protein Information: transcription factor RelB
• UniProt Protein Name: Transcription factor RelB
• Protein Family: Transcription factor
• UniProt Gene Name: Relb
• UniProt Entry Name: RELB_MOUSE

Uniprot Description

RELB: a transcription factor of the nuclear factor-kappaB (NFkB) transcription complex. Stimulates promoter activity in the presence NFkB1/NFkB-p50. Plays role in regulation of apoptosis in hepatocellular carcinoma through activation of downstream target genes. Does not associate with DNA or RelA/NFkB-p65.

Protein type: Transcription factor

Cellular Component: cytoplasm; cytoskeleton; cytosol; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; protein binding; transcription factor activity

Biological Process: antigen processing and presentation; circadian regulation of gene expression; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; myeloid dendritic cell differentiation; negative regulation of interferon-beta production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to cytokine stimulus; rhythmic process; T-helper 1 cell differentiation; T-helper 1 type immune response; transcription, DNA-dependent