IgG Fc Secondary Antibody | IgG secondary antibody
Goat anti-Human IgG Fc, HRP, AffiPure
Gene Names
FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Synonyms
IgG Fc; Goat anti-Human IgG Fc; HRP; AffiPure; HRP Goat Anti-Human IgG Fc; IgG secondary antibody
Sequence Length
315
Preparation and Storage
Stored at 2-8 degree C for 24 months.
Related Product Information for IgG secondary antibody
Description: Goat Anti-Human IgG recognizes human IgG F(c) fragment. This HRP-conjugated secondary antibody was purified using antigen affinity chromatography and is suitable for ELISA and Western blotting techniques. The IgG component of this conjugate is specific for normal human IgG and is non-reactive with k or l light chains. The IgG-HRP conjugate contains between 2 and 4 HRP molecules per IgG molecule.
Background: HRP Goat Anti Human IgG Fc is suitable for ELISA, Western blotting, and histology techniques.
Goats were immunized with a purified preparation of human IgG Fc fragment that was obtained from a purified normal human IgG antibody protein pool. The hyperimmune IgG fraction was initially solid phase adsorbed to obtain class specificity. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled human IgG Fc protein.
Affinity purified, anti-human IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-human IgG Fc prep just prior to its reaction with a 4-fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule.
Using ID, IEP, and ELISA analysis techniques, the IgG component of this conjugate was specific for normal human IgG and was non-reactive with k or l light chains. This conjugate is suitable for ELISA and Western blotting techniques.
The conjugate is dissolved in a Tris based conjugate stabilization buffer containing 50 ppm ProClin 300 anti-microbial agent.
Background: HRP Goat Anti Human IgG Fc is suitable for ELISA, Western blotting, and histology techniques.
Goats were immunized with a purified preparation of human IgG Fc fragment that was obtained from a purified normal human IgG antibody protein pool. The hyperimmune IgG fraction was initially solid phase adsorbed to obtain class specificity. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled human IgG Fc protein.
Affinity purified, anti-human IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-human IgG Fc prep just prior to its reaction with a 4-fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule.
Using ID, IEP, and ELISA analysis techniques, the IgG component of this conjugate was specific for normal human IgG and was non-reactive with k or l light chains. This conjugate is suitable for ELISA and Western blotting techniques.
The conjugate is dissolved in a Tris based conjugate stabilization buffer containing 50 ppm ProClin 300 anti-microbial agent.
Product Categories/Family for IgG secondary antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
230-310 kDa
NCBI Official Full Name
IgG Fc receptor
NCBI Official Synonym Full Names
Fc fragment of IgG receptor IIa
NCBI Official Symbol
FCGR2A
NCBI Official Synonym Symbols
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-a
NCBI Description
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
Research Articles on IgG
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Product Notes
The IgG (Catalog #AAA258084) is a Secondary Antibody and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IgG Fc, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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