Rat BDNF (Brain Derived Neurotrophic Factor) CLIA Kit | BDNF clia kit
Rat BDNF (Brain Derived Neurotrophic Factor) CLIA Kit
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to BDNF. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of BDNF. You can calculate the concentration of BDNF in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it.