Thyroglobulin Native Protein | TG native protein

Native Thyroglobulin (TG)

Cat. Num. AAA2032917

• Gene Names: TG; TGN; AITD3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Thyroglobulin; Native Thyroglobulin (TG); AITD3; TGN; TG native protein

• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence Length: 2768

• Applicable Applications for TG native protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Relevance: Thyroglobulin (TG) is a 660 kDa, dimeric protein produced by the follicular cells of the thyroid and used entirely within the thyroid gland. Thyroglobulin protein accounts for approximately half of the protein content of the thyroid gland. And, TG is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). Moreover, thyroglobulin levels in the blood are mainly used as a tumor marker for certain kinds of thyroid cancer (particularly papillary or follicular thyroid cancer). Thyroglobulin is not produced by medullary or anaplastic thyroid carcinoma.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Organism Species: Bovine
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 55770862
• NCBI GeneID: 7038
• NCBI Accession #: NP_003226.4
• NCBI GenBank Nucleotide #: NM_003235.4
• Molecular Weight: 298,383 Da
• NCBI Official Full Name: thyroglobulin
• NCBI Official Synonym Full Names: thyroglobulin
• NCBI Official Symbol: TG
• NCBI Official Synonym Symbols: TGN; AITD3
• NCBI Protein Information: thyroglobulin
• UniProt Protein Name: Thyroglobulin
• Protein Family: Thyroglobulin
• UniProt Gene Name: TG
• UniProt Synonym Gene Names: Tg
• UniProt Entry Name: THYG_HUMAN

NCBI Description

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]

Uniprot Description

TG: Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Defects in TG are the cause of thyroid dyshormonogenesis 3 (TDH3). A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases. Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3). AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus; perinuclear region of cytoplasm; protein complex

Molecular Function: anion binding; chaperone binding; hormone activity; protein complex binding; protein homodimerization activity

Biological Process: hormone biosynthetic process; iodide transport; regulation of myelination; response to lipopolysaccharide; response to pH; signal transduction; thyroid gland development; thyroid hormone metabolic process; transcytosis

Disease: Autoimmune Thyroid Disease, Susceptibility To, 3; Thyroid Dyshormonogenesis 3