Vitamin D Binding Protein (DBP) Recombinant Protein | DBP recombinant protein

Recombinant Vitamin D Binding Protein (DBP)

Cat. Num. AAA2011207

• Gene Names: Gc; DBP; VDB
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vitamin D Binding Protein (DBP); Recombinant Vitamin D Binding Protein (DBP); DBP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-QLTSFI EKGQEMCADY SENTFTEYKK KLAERLRTKT PNTSPAELKD MVEKHSDFAS KCCSINSPPL YCSSQIDAEM IDT
• Sequence Length: 476

• Applicable Applications for DBP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gln395~Thr473 (Accession # P21614) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 51172612
• NCBI GeneID: 14473
• NCBI Accession #: NP_032122.1
• NCBI GenBank Nucleotide #: NM_008096.2
• UniProt Accession #: P21614; Q7TS97; Q91XG1; Q9CY31
• Molecular Weight: 10.6kDa
• NCBI Official Full Name: vitamin D-binding protein
• NCBI Official Synonym Full Names: group specific component
• NCBI Official Symbol: Gc
• NCBI Official Synonym Symbols: DBP; VDB
• NCBI Protein Information: vitamin D-binding protein; gc-globulin
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Remorin
• UniProt Gene Name: Gc
• UniProt Synonym Gene Names: DBP; VDB
• UniProt Entry Name: VTDB_MOUSE

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; axon; perinuclear region of cytoplasm; extracellular region; cytosol

Molecular Function: vitamin D binding; actin binding; vitamin transporter activity

Biological Process: transport; vitamin transport; vitamin D metabolic process