Mouse SNRPN Monoclonal Antibody | anti-SNRPN antibody

Anti-SNRPN Antibody (Monoclonal, 6F12)

Cat. Num. AAA1752177

• Gene Names: SNRPN; SMN; PWCR; SM-D; sm-N; RT-LI; HCERN3; SNRNP-N; SNURF-SNRPN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: SNRPN; Monoclonal Antibody; Anti-SNRPN Antibody (Monoclonal; 6F12); Small nuclear ribonucleoprotein-associated protein N; small nuclear ribonucleoprotein polypeptide N; snRNP-N; Sm protein D; Sm-D; Sm protein N; Sm-N; SmN; Tissue-specific-splicing protein; HCERN3; SMN; anti-SNRPN antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized; Contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 244

• Applicable Applications for anti-SNRPN antibody: Western Blot (WB)
• Application Notes: WB: 0.1-0.5 mug/ml; Tested Species: In-house tested species with positive results.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SNRPN (11-39aa QHIDYRMRCILQDGRIFIGTFKAFDKHMN), identical to the related mouse and rat sequences.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500mug/ml.; Relevant Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month.; It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-SNRPN antibody

Description: Mouse IgG monoclonal antibody for SNRPN detection. Tested with WB in Human; Mouse; Rat.
Background: SNRPN(Small Nuclear Ribonucleoprotein Polypeptide N), also called SMN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide. The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. SNRPN also encodes a long alternatively spliced transcript containing several small nucleolar RNAs (snoRNAs) and extends downstream to partially overlap the UBE3A gene in the antisense orientation. PWS arises from loss of function of genes in this region expressed exclusively from the paternal chromosome, suggesting that SNRPN may play a role in its etiology. The SNRPN gene is mapped on 15q11.2. Analysis of maternal DNA and of SNRPN cDNA confirmed that the maternal allele is not expressed in fetal brain and heart. Deletions in the transcription unit of the imprinted SNRPN gene occur in patients who have PWS or Angelman syndrome because of a parental imprint switch failure in this chromosomal domain. 

References

1. Bielinska, B., Blaydes, S. M., Buiting, K., Yang, T., Krajewska-Walasek, M., Horsthemke, B., Brannan, C. I. De novo deletions of SNRPN exon 1 in early human and mouse embryos result in a paternal to maternal imprint switch. Nature Genet. 25: 74-78, 2000. 2. Cattanach, B. M., Barr, J. A., Evans, E. P., Burtenshaw, M., Beechey, C. V., Leff, S. E., Brannan, C. I., Copeland, N. G., Jenkins, N. A., Jones, J. A candidate mouse model for Prader-Willi syndrome which shows an absence of Snrpn expression. Nature Genet. 2: 270-274, 1992. 3. Geuns, E., De Rycke, M., Van Steirteghem, A., Liebaers, I. Methylation imprints of the imprint control region of the SNRPN-gene in human gametes and preimplantation embryos. Hum. Molec. Genet. 12: 2873-2879, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 4507135
• NCBI GeneID: 6638
• NCBI Accession #: NP_003088.1
• NCBI GenBank Nucleotide #: NM_003097.5
• UniProt Accession #: P63162; P14648; P17135; Q0D2Q5; B3KVR1
• Molecular Weight: 25,076 Da
• NCBI Official Full Name: small nuclear ribonucleoprotein-associated protein N
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptide N
• NCBI Official Symbol: SNRPN
• NCBI Official Synonym Symbols: SMN; PWCR; SM-D; sm-N; RT-LI; HCERN3; SNRNP-N; SNURF-SNRPN
• NCBI Protein Information: small nuclear ribonucleoprotein-associated protein N
• UniProt Protein Name: Small nuclear ribonucleoprotein-associated protein N
• Protein Family: SNRPN upstream reading frame protein
• UniProt Gene Name: SNRPN
• UniProt Synonym Gene Names: HCERN3; SMN; snRNP-N; Sm-D; Sm-N; SmN

NCBI Description

This gene is located within the Prader-Willi Syndrome critical region on chromosome 15 and is imprinted and expressed from the paternal allele. It encodes a component of the small nuclear ribonucleoprotein complex, which functions in pre-mRNA processing and may contribute to tissue-specific alternative splicing. Alternative promoter use and alternative splicing result in a multitude of transcript variants encoding the same protein. Transcript variants that initiate at the CpG island-associated imprinting center may be bicistronic and also encode the SNRPN upstream reading frame protein (SNURF) from an upstream open reading frame. In addition, long spliced transcripts for small nucleolar RNA host gene 14 (SNHG14) may originate from the promoters at this locus and share exons with this gene. Alterations in this region are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]

Uniprot Description

May be involved in tissue-specific alternative RNA processing events.