Proteasome 26S Subunit Recombinant Protein | PSMD11 recombinant protein

Recombinant Human Proteasome 26S Subunit, Non-ATPase 11

Cat. Num. AAA145635

• Gene Names: PSMD11; S9; Rpn6; p44.5
• Purity: Greater than 85% as determined by SDS-PAGE.
• Synonyms: Proteasome 26S Subunit; Recombinant Human Proteasome 26S Subunit; Non-ATPase 11; PSMD11 Human; Non-ATPase 11 Human Recombinant; S9; Rpn6; p44.5; MGC26S proteasome regulatory subunit S9; 26S proteasome regulatory subunit p44.5; PSMD11.3844; 26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit RPN6; PSMD11 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85% as determined by SDS-PAGE.
• Form/Format: The PSMD11 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAVVEFQ RAQSLLSTDR EASIDILHSI VKRDIQENDE EAVQVKEQSI LELGSLLAKT GQAAELGGLL KYVRPFLNSI SKAKAARLVR SLLDLFLDME AATGQEVELC LECIEWAKSE KRTFLRQALE ARLVSLYFDT KRYQEALHLG SQLLRELKKM DDKALLVEVQ LLESKTYHAL SNLPKARAAL TSARTTANAI YCPPKLQATL DMQSGIIHAA EEKDWKTAYS YFYEAFEGYD SIDSPKAITS LKYMLLCKIM LNTPEDVQAL VSGKLALRYA GRQTEALKCV AQASKNRSLA DFEKALTDYR AELRDDPIIS THLAKLYDNL LEQNLIRVIE PFSRVQIEHI SSLIKLSKAD VERKLSQMIL DKKFHGILDQ GEGVLIIFDE PPVDKTYEAA LETIQNMSKV VDSLYNKAKK LT
• Sequence Length: 422

Related Product Information for PSMD11 recombinant protein

Description: PSMD11 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 442 amino acids (1-422a.a) and having a molecular mass of 49.6kDa. PSMD11 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: The 26S proteasome is a multicatalytic proteinase complex with a well ordered structure composed of two complexes- a 20S core and a 19S regulator. PSMD11 is a non-ATPase subunit of the 19S regulator. The 20S core is composed of four rings of 28 non-identical subunits; two rings are composed of 7 alpha subunits and two rings are composed of 7 beta subunits. The 19S regulator is composed of a base that contains six ATPase subunits and two non-ATPase subunits, and a lid that contains up to ten non-ATPase subunits. Proteasomes are spread throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An indispensable function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.

Product Categories/Family for PSMD11 recombinant protein

Enzymes; Proteasome

NCBI and Uniprot Product Information

• NCBI GI #: 394953908
• NCBI GeneID: 5717
• NCBI Accession #: NP_001257411.1
• NCBI GenBank Nucleotide #: NM_001270482.1
• UniProt Accession #: O00231; O00495; Q53FT5; A8K3I7; E1P663
• Molecular Weight: 47,535 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 11
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
• NCBI Official Symbol: PSMD11
• NCBI Official Synonym Symbols: S9; Rpn6; p44.5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit 9; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; 26S proteasome regulatory subunit p44.5
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 11
• UniProt Gene Name: PSMD11
• UniProt Entry Name: PSD11_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMD11: Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Belongs to the proteasome subunit S9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; proteasome complex; membrane; proteasome regulatory particle; cytosol; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; stem cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; proteasome assembly; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis