Cytochrome P450 90B1 (CYP90B1) Recombinant Protein | CYP90B1 recombinant protein

Recombinant Arabidopsis thaliana Cytochrome P450 90B1 (CYP90B1) , partial

• Gene Names: DWF4; AtDWF4; CLM; CLOMAZONE-RESISTANT; CYP90B1; CYTOCHROME P450 90B1; DWARF 4; PARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1; PARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1; PSC1; SAV1; SHADE AVOIDANCE 1; SNP2; STEROID 22-ALPHA-HYDROXYLASE; SUPPRESSOR OF
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Cytochrome P450 90B1 (CYP90B1); Recombinant Arabidopsis thaliana Cytochrome P450 90B1 (CYP90B1); partial; CYP90B1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 513

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 15229822
• NCBI GeneID: 824229
• NCBI Accession #: NP_190635.1
• NCBI GenBank Nucleotide #: NM_114926.4
• UniProt Accession #: O64989; Q9SCQ9
• Molecular Weight: 58,868 Da
• NCBI Official Full Name: Cytochrome P450 superfamily protein
• NCBI Official Symbol: DWF4
• NCBI Official Synonym Symbols: AtDWF4; CLM; CLOMAZONE-RESISTANT; CYP90B1; CYTOCHROME P450 90B1; DWARF 4; PARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1; PARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1; PSC1; SAV1; SHADE AVOIDANCE 1; SNP2; STEROID 22-ALPHA-HYDROXYLASE; SUPPRESSOR OF
• NCBI Protein Information: Cytochrome P450 superfamily protein
• UniProt Protein Name: Cytochrome P450 90B1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP90B1
• UniProt Synonym Gene Names: DWF4; Dwarf4

NCBI Description

Encodes a 22alpha hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway. The protein is a member of CYP90B gene family. CLM is an epi-allele with small, compressed rosette, reduced internode length, and reduced fertility, appears in selfed ddm mutant plants possibly due to loss of cytosine methylation. Transcripts accumulate in actively growing tissues, and GUS expression is negatively regulated by brassinosteroids. Localized in the endoplasmic reticulum. The in vitro expressed protein can perform the C-22 hydroxylation of a variety of C27-, C28- and C29-sterols. Cholesterol was the best substrate, followed by campesterol. Sitosterol was a poor substrate.

Uniprot Description

Catalyzes the C22-alpha-hydroxylation step in brassinosteroids biosynthesis. Converts campestanol to 6-deoxocathasterone and 6-oxocampestanol to cathasterone.